Phlebotomy Order Of Draw Study (Nhco
July 2024) Questions And Answers With
Verified Solutions Already Passed!!!
Lab responsible for blood cultures? - ANSWER✔✔Microbiology
How and what do you clean for blood culture? - ANSWER✔✔ChloraPrep (not alc)
at injection site and wipe down the tops of collection bottles
What order do you fill blood culture bottles? - ANSWER✔✔Aerobic first and then
anaerobic (butterfly tubing contains air, hence why aerobic is first)
What does STAT mean? - ANSWER✔✔Test must be carried out immediately
T/F) Blood cultures are usually ordered STAT or as timed specimens -
ANSWER✔✔True
What is the purpose of sodium polyanethole sulfonate (SPS)? - ANSWER✔✔To
promote rapid growth of pathogenic/infectious microorganisms in a blood sample
(for identification)- blood culture bottles
What tubes come before and after blood cultures? - ANSWER✔✔No tubes before,
discard tube after (clear or glass red top).
,What needle should you use for a blood culture? - ANSWER✔✔Use a winged
infusion set (butterfly) to prevent nutrient broth introduction to blood stream (may
differ by employer)
Which red top tube (glass/plastic) do you use as a discard? - ANSWER✔✔Glass
(glass red tops contain no additives)
What is a discard tube used for? - ANSWER✔✔Used to prevent thrombin and
other cells from polluting the samples drawn in following tubes.
What tubes come before and after a discard tube? - ANSWER✔✔Blood culture
bottles before, light blue top tube after
What tubes come before and after a light blue top? - ANSWER✔✔Discard tube
before (clear, red glass), royal blue with red stripe after
T/F) the royal blue with red stripe tube has no additives - ANSWER✔✔True
Does the royal blue red stripe tube yield serum or plasma? - ANSWER✔✔Serum
What is the difference between a glass red top tube (no additive) and a royal blue
red stripe tube? - ANSWER✔✔The royal blue red stripe tube contains no nickel
What is the difference between a purple top tube and a royal blue lavender/blue
stripe tube? - ANSWER✔✔The royal blue lavender/blue stripe tube contains no
nickel
, What 2 tests are royal blue tubes used for? (Red stripe or lavender/blue stripe) -
ANSWER✔✔Lead (Pb) poisoning, Heavy metal toxicology
Lab responsible for royal blue tubes? (Red stripe or lavender/blue stripe) -
ANSWER✔✔Toxicology
How many times should you invert royal blue tube? (Red stripe or lavender/blue
stripe) - ANSWER✔✔8 times (but you will invert all tubes 8-10 times)
What tubes come before and after royal blue red stripe tube? - ANSWER✔✔Light
blue before, plastic red top after
What tubes come before and after royal blue lavender/blue stripe tube? -
ANSWER✔✔Lavender before, grey after
Lab responsible for light blue top tube? - ANSWER✔✔Coagulation (or
Hematology if a smaller facility)
Additive in light blue tube? What does it do? - ANSWER✔✔Sodium citrate-
anticoagulant (prevents blood from coagulating and using up coagulation factors
before reaching the lab)
How does sodium citrate work? - ANSWER✔✔Sodium citrate prevents
coagulation by binding to the calcium in a specimen. This preserves the specimens
coagulation factors.
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