BIO 354 Cell Bio Lab Exam | Questions and Answers Imagine you are a scuba diver the size of a virus exploring huge algae cells. If you wanted to see how the water molecules are split inside the thylakoids space, how many membranes would you have to cross to get there? 3 You would go through the ...
Imagine you are a scuba diver the size of a virus exploring huge algae cells. If you
wanted to see how the water molecules are split inside the thylakoids space, how many
membranes would you have to cross to get there?
3
You would go through the cell wall, to get the plasma membrane then you would be
inside the cytoplasm. From there you would swim to the chloroplast and cross the outer
and I. Er membranes to get the storms, where you'd see colorful stacks of thylakoids.
Where are the pigment molecules held in place?
By the photosystem, a protein complex.
Why do pigments absorb visible light and other molecules don't
Extended conjugated systems
Extended conjugated systems are alternating double bonds and/or atoms with a free
electron pair. Usually nitrogen and oxygen
Chloroplast are suspended in an isolation buffer. To be able to extract the pigments
from the chloroplast, we have to get rid of the first buffer first. What's the best way to
separate the chloroplast from the buffer
Centrifugation.
Inside the spinning centrifuge, the sample is separated according to the densities if the
particles
To elite all the pigment contained in the chloroplasts, what do you have to consider,
when choosing from different solvents?
Use both hydrophobic and hydrophilic solvents
What color is not absorbed by hexane according to the absorbance spectrum? What
kind of pigment is this?
Green, chlorophyll
What color does the water extract absorb
Green
Where does the light reaction take place?
thylakoid membrane
, Where does the Calvin cycle take place?
stroma of the chloroplast
Where is the light energy converted into electron flow
In the reaction center chlorophyll
In what order do he electrons move through the electron transport chain
Water, photosystem II, photosystem I, NADP
What is produced during the light reaction
O2 + 2 NADPH + 2H+ + nATP
With DCPIP we measure the electron flow from...
Photosystem II
What would be a good positive control for the photosystem II experiment
Expose a sample to regular light
What would be the best negative control for the photosystem II experiment
Keeping the sample in the dark
What wavelength should the chlorophyll not be absorbed
580nm
If the red pigment is able to transfer the excitation energy to the reaction center, what
would you expect to happen
To see a decrease in amount of oxidized (blue) DCPIP
Why do we use DCMU
Avoid the redox for DCPIP. DCMU disables the electron transport chain by blocking the
binding site of photosystem II
What wavelength should we use to measure the amount of oxidized DCPIP?
610
Is red pigment really a photosynthetic pigment?
Yes, because photosystem 2 is active in green light
Most important finding is photosystem II lab
Red pigment can be used to block photosynthesis
Metabolism can be divided into catabolic and anabolic reactions. How are these
connected?
Catabolic reactions generate energy that anabolic reactions use
How many carbon atoms does one molecule of pyruvate contain
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