NWHSU Microbiology - Lab Final Exam Questions Verified Answers Current Update (A+ Pass)
5 views 0 purchase
Course
NWHSU
Institution
NWHSU
NWHSU Microbiology - Lab Final Exam Questions Verified Answers Current Update (A+ Pass)
Define titer - Answers - The highest dilution of a sample that tests positive for an antibody.
Which immunoglobulin appears first when someone has an infection? - Answers - IgM
Define Prozone. - Answers...
NWHSU Microbiology - Lab Final Exam
Questions Verified Answers Current
Update (A+ Pass)
Define titer - Answers -✔✔ The highest dilution of a sample that tests positive for an
antibody.
Which immunoglobulin appears first when someone has an infection? - Answers -✔✔
IgM
Define Prozone. - Answers -✔✔ During antibody precipitation tests, its the period of
time when antibody concentrations are high and no precipitates are formed, leading to
false negatives.
How is a positive Western blot for HIV determined? - Answers -✔✔ There will be two or
more dark bands.
what are the reagents in gram staining? - Answers -✔✔ Crystal violet, Grams iodine,
acetone-alcohol, safranin.
In gram staining, what does crystal violet do? - Answers -✔✔ stains all gram + and -
bacteria.
In gram staining, what does grams iodine do? - Answers -✔✔ its a mordant that forms
violet-iodine complex that binds more.
In gram staining, what does acetone-alcohol do? - Answers -✔✔ It decolorizes gram -
bacteria. But only if not done for too long.
In gram staining, what does safranin do? - Answers -✔✔ counterstains gram - bacteria.
What color are gram positive bacteria? - Answers -✔✔ Purple
What color are gram negative bacteria? - Answers -✔✔ pink
How do gram stains and wet mounts vary? - Answers -✔✔ Gram can determine gram
+/-. Wet mounts can tell motility.
How are gram stains and wet mounts similar? - Answers -✔✔ They both can see
arrangement, size, and shape.
, What is the KOH test to differentiate gram positive from gram negative bacteria? -
Answers -✔✔ Mix KOH with a colony for 20 seconds. Stringy=negative. Not
stringy=positive.
What are 3 spore forming bacteria that cause disease? - Answers -✔✔ Bacillus
Anthracis, Clostridium tetani, Clostridium botulinum.
What bacteria causes anthrax? - Answers -✔✔ Bacillus Anthracis
WHat bacteria causes Lock jaw - Answers -✔✔ Clostridium tetani
What bacteria causes botulism - Answers -✔✔ Clostridium botulinum
Define alpha hemolysis and what does it look like? - Answers -✔✔ Bacteria that can
partiatlly break down heme pigment (green halo)
Define beta hemolysis and what does it look like? - Answers -✔✔ bacteria that can
break down heme pigment fully. (clear zone)
Define gamma hemolysis and what does it look like? - Answers -✔✔ bacteria that show
no change to a blood agar plate.
Which hemolysis is a lab looking for in diagnosing strept throat? - Answers -✔✔ beta
hemolysis
What is the importance of a capsule? - Answers -✔✔ It protects against microphages
in lungs.
What stain shows a capsule? - Answers -✔✔ gin stain.
what is the name of the acid-fast stain and what is it used for? - Answers -✔✔ Ziehl-
Neelson procedure uses carbol fushsin. It detects bacteria with cell walls rich in lipids
and waxes. LIke Mycobacterium and Nocardia. Leprosy, TB,
Define selective media? - Answers -✔✔ Isolates/cultivates for specific bacteria
Define differential media? - Answers -✔✔ Shows chemical reactions of bacteria
Is MacConkeys selective or Differential? - Answers -✔✔ Both
Is blood agar selective or differential? - Answers -✔✔ Differential
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Stuviaascorers. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $12.99. You're not tied to anything after your purchase.
