BIO 353 Study Guide Exam 1 Questions And Answers Graded A+.
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Course
BIO 353
Institution
BIO 353
BIO 353 Study Guide Exam 1 Questions And Answers Graded A+.
The cell - correct answer. The basic unit of structure and function in living things
What are the major Organelles/ structures of a basic eukaryotic cell? - correct answer. nucleus
nucleolus
ribosomes
smooth ER
rough ...
BIO 353 Study Guide Exam 1 Questions
And Answers Graded A+.
The cell - correct answer. The basic unit of structure and function in living things
What are the major Organelles/ structures of a basic eukaryotic cell? - correct answer.
nucleus
nucleolus
ribosomes
smooth ER
rough ER
mitochondria
What are the 3 major disciplines involved with studying biology? - correct answer.
bioimaging
biochemistry
mol. biology/genetics
What is light microscopy? - correct answer. visible light passes through a series of
lenses to produce a magnified image
What is the resolution limit of the light microscope? - correct answer. .2 nm
Define resolution - correct answer. Describes the degree of clarity, detail, and
sharpness of an image
Define magnification - correct answer. the ratio of an object's image size to its real
size
what is one of the major advantages of the basic light microscope? - correct answer.
ability to image LIVE cells
, State of cells when using amplitude contrast - correct answer. fixed/dead
Describe amplitude contrast - correct answer. a form of contrast generated by the
absorption of selective wave lengths from the visible light spectrum
Describe phase contrast microscopy - correct answer. dependent on light refraction
contrast formed b/c different parts have different densities
State of cells when using phase contrast? - correct answer. living
epifluorescence microscopy - correct answer. detection of specific
molecules/ions/organelles that are MADE fluorescent by coupling them to a
flourochrome marker
indirect immunofluorescence - correct answer. i. cells/tissues fixed
ii. exposed to primary antibody, antibody binds to specific protein (ie antigen)
iii. exposed to secondary antibody that is coupled to a flourochrome marker
What is GFP and how is it coupled to the proteins in different cellular structures (direct
method)? - correct answer. Green Fluorescent Protein binds the flourescent sntibody
to the corresponding antigen on the target protein
Whats the advantage of using GFP? - correct answer. cells are ALIVE
What are vital fluorescent dyes (direct method)? - correct answer. harmless dyes used
to stain living tissue for observation
confocal microscopy - correct answer. a light microscope that uses fluorescent stains
and laser to make two- and three-dimensional images
Advantages of confocal microscopy - correct answer. increased clarity of images and
resolution
What is electron microscopy? - correct answer. technique used for obtaining high
resolution images of specimens
transmission electron microscope - correct answer. used to study the internal
structure of cells
Advantage of electron microscope - correct answer. shorter wavelength of electron
beam= increased resolution and magnification
Disadvantage of electron microscopes - correct answer. cannot view living things- life
is not compatible with vacuum
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