UConn MCB 2610 Final Lab Practical Questions And Answers Latest Updates
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Course
UConn MCB 2610
Institution
UConn MCB 2610
Which of the following is an indirect mode of transmission - an insect, a cellphone, a
sneeze? - ️️Cellphone
True/False: malachite green stains the vegetative cells green, while the safranin stains
endospores pink. - ️️FALSE
The ________ method is a quick and easy way to isolate bacteri...
UConn MCB 2610 Final Lab Practical
Which of the following is an indirect mode of transmission - an insect, a cellphone, a
sneeze? - ✔️✔️Cellphone
True/False: malachite green stains the vegetative cells green, while the safranin stains
endospores pink. - ✔️✔️FALSE
The ________ method is a quick and easy way to isolate bacteria. - ✔️✔️Streak plate
How many times do you get culture from the tube while performing a t-streak? -
✔️✔️Once
Who do you contact to reschedule a lab? - ✔️✔️Gino
The culture tubes are disposed of in the gray trash bin. True/false? - ✔️✔️FALSE
What is the first thing you do after a spill? - ✔️✔️Tell the TA
What is the last thing you do before leaving lab? - ✔️✔️Wash hands for 20 seconds
The outer cone is the hottest part of the flame. True/false? - ✔️✔️FALSE
Where are slides disposed of? - ✔️✔️Sharps container
What must you do to the loop before every use? - ✔️✔️Heat in Bunsen-burner flame
True/False: When making a smear of the culture, you heat fix the slide before air drying
it completely. - ✔️✔️FALSE
True/False: wiping down the bench with wescodyne is the last thing you do when
leaving the lab. - ✔️✔️FALSE
What is the magnification value of the ONLY objective lens that should come in contact
with immersion oil? - ✔️✔️100x
Pertaining to Gram stain, cells that retain crystal violet and appear purple are said to
be_____. - ✔️✔️Gram positive
True/False: your name, the date, and sample information goes on the lid of the agar
dish. - ✔️✔️FALSE
, Pertaining to Gram stain, cells that do not retain crystal violet and appear pink, are said
to be ____. - ✔️✔️Gram negative
True/False: heat used during staining causes sporulation. - ✔️✔️FALSE
True/False: Gram stains and spore stains are examples of simple stains. - ✔️✔️FALSE
What causes bacterial endospore formation? - ✔️✔️Response to environmental
conditions, i.e., lack of nutrients
What are the 4 phases of microbial growth? - ✔️✔️Lag, exponential/log, stationary,
death
True/False: we will measure the optical density over time to determine growth rate. -
✔️✔️TRUE
Define generation time. - ✔️✔️Time for population to double
What does it mean for a culture to be in stationary phase? - ✔️✔️Rate of replication
matches rate of death
True/False: you can use oil with the 40x objective lens. - ✔️✔️FALSE
True/False: generation time is calculated in the log phase. - ✔️✔️TRUE
What does it mean for a culture to be in log phase? - ✔️✔️Rapid growth and
replication; each cell doubles
True/False: Gram positive bacteria have a thick peptidoglycan layer which retains
safranin and appears pink. - ✔️✔️FALSE
True/False: the purpose of heating during the spore stain is to create an endospore. -
✔️✔️FALSE
What group of organisms does MacConkey agar select against? - ✔️✔️Gram positive
True/False: complex media is made with a precise chemical composition. -
✔️✔️FALSE
What organism does SF broth select for? - ✔️✔️Enterococcus faecalis
What type of bacteria can be differentiated using an EnteroPluri tube? - ✔️✔️Enteric
family of bacteria encompassing gram negative, non-sporeforming, facultative
anaerobic rods
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