EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
MB (ASCP) Exam Questions and
Answers |100% Pass
Predominant form of DNA - Answer✔✔-Right-handed B
DNA and histone interaction is due to what type of bond? - Answer✔✔-Ionic
What stains to the minor groove of DNA duplex? - Answer✔✔-SYBR Green
What is the most abundant type of RNA? - Answer✔✔-Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - Answer✔✔-Genome and mRNA
What increases half-life of mRNA? - Answer✔✔-5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Answer✔✔-Euchromatin
What is the most common type of eukaryotic DNA? - Answer✔✔-Satellite DNA
What specimen has the most DNA according to molecular weight? - Answer✔✔-White Blood
Cells
What amino acids compose histones? - Answer✔✔-Lysine and Arginine
Amino acid that all proteins start with: - Answer✔✔-Methionine
What protects the DNA strand during replication? - Answer✔✔-Single-stranded binding protein
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,EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
Leading Strand - Answer✔✔-3' to 5' template
Lagging Strand - Answer✔✔-5' to 3' template
What DNA strand is being copied in transcription? - Answer✔✔-Antisense Strand
First step after newly formed mRNA is made? - Answer✔✔-Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Answer✔✔-Telomerase
What polymerase has repair activity? - Answer✔✔-Beta/Epsilon
What polymerase acts on leading strand? - Answer✔✔-Delta
What polymerase acts on lagging strand? - Answer✔✔-Alpha
Which polymerase is used for mitochondrial replication and repair? - Answer✔✔-Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔✔-Topoisomerase
Which RNA polymerase is mainly for transcription? - Answer✔✔-RNA Pol II
Which polymerase is involved with mRNA synthesis? - Answer✔✔-RNA Pol II
What inactivates RNAses? - Answer✔✔-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to
6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Answer✔✔-Restriction Enzyme
Type II
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,EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
What type of restriction enzyme lacks methylation activity? - Answer✔✔-Restriction Enzyme
Type II
What is necessary to make cDNA? - Answer✔✔-Reverse transcriptase
Number of SNPs in a human genome? - Answer✔✔-11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Answer✔✔-3 billion base pairs
The polymerization of acrylamide requires: - Answer✔✔-Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? - Answer✔✔-Inversely proportional
What is denatured during a southern blot? - Answer✔✔-Hydrogen bonds (inter-strand bonds)
Melting temperature formula - Answer✔✔-Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - Answer✔✔-5' G
Proper GC content of primer - Answer✔✔-35-65%
Inhibitors of PCR - Answer✔✔-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol
Blue
Order to add to a PCR reaction: - Answer✔✔-Master mix > patient sample > positive control >
negative control
Pre Area - Answer✔✔-Positive Pressure
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, EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
Post Area - Answer✔✔-Negative Pressure
Which PCR requires two steps for specificity? - Answer✔✔-Nested PCR
Which microorganism is detected by branched DNA amplification? - Answer✔✔-DNA or RNA
Which technique uses RNA, antibodies, and DNA? - Answer✔✔-Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation - Answer✔✔-Bisulfite
DNA
What tests are isothermal? - Answer✔✔-NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? - Answer✔✔-AML
T-cell gene rearrangement is seen in what malignancy? - Answer✔✔-Sezary Syndrome
Which translocation causes Acute Premyelocytic Leukemia? - Answer✔✔-t 15;17
Ewing Sarcoma - Answer✔✔-t 11;22
Synovial Sarcoma - Answer✔✔-t X;18
Follicular Lymphoma - Answer✔✔-t 14;18
What genes would be screened in a colorectal panel? - Answer✔✔-MSH2 & MLH1
What gene mutation causes a high risk of breast or ovarian cancer? - Answer✔✔-BRCA1 and
BRCA2
What does MSI test for? - Answer✔✔-Mutations in mismatch repair genes (MMR)
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MB (ASCP) Exam Questions and
Answers |100% Pass
Predominant form of DNA - Answer✔✔-Right-handed B
DNA and histone interaction is due to what type of bond? - Answer✔✔-Ionic
What stains to the minor groove of DNA duplex? - Answer✔✔-SYBR Green
What is the most abundant type of RNA? - Answer✔✔-Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - Answer✔✔-Genome and mRNA
What increases half-life of mRNA? - Answer✔✔-5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Answer✔✔-Euchromatin
What is the most common type of eukaryotic DNA? - Answer✔✔-Satellite DNA
What specimen has the most DNA according to molecular weight? - Answer✔✔-White Blood
Cells
What amino acids compose histones? - Answer✔✔-Lysine and Arginine
Amino acid that all proteins start with: - Answer✔✔-Methionine
What protects the DNA strand during replication? - Answer✔✔-Single-stranded binding protein
Page 1/33
,EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
Leading Strand - Answer✔✔-3' to 5' template
Lagging Strand - Answer✔✔-5' to 3' template
What DNA strand is being copied in transcription? - Answer✔✔-Antisense Strand
First step after newly formed mRNA is made? - Answer✔✔-Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
Answer✔✔-Telomerase
What polymerase has repair activity? - Answer✔✔-Beta/Epsilon
What polymerase acts on leading strand? - Answer✔✔-Delta
What polymerase acts on lagging strand? - Answer✔✔-Alpha
Which polymerase is used for mitochondrial replication and repair? - Answer✔✔-Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔✔-Topoisomerase
Which RNA polymerase is mainly for transcription? - Answer✔✔-RNA Pol II
Which polymerase is involved with mRNA synthesis? - Answer✔✔-RNA Pol II
What inactivates RNAses? - Answer✔✔-Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to
6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Answer✔✔-Restriction Enzyme
Type II
Page 2/33
,EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
What type of restriction enzyme lacks methylation activity? - Answer✔✔-Restriction Enzyme
Type II
What is necessary to make cDNA? - Answer✔✔-Reverse transcriptase
Number of SNPs in a human genome? - Answer✔✔-11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Answer✔✔-3 billion base pairs
The polymerization of acrylamide requires: - Answer✔✔-Catalyst (APS or TEMED) or Light
activation
How does agarose gel pore size relate to concentration? - Answer✔✔-Inversely proportional
What is denatured during a southern blot? - Answer✔✔-Hydrogen bonds (inter-strand bonds)
Melting temperature formula - Answer✔✔-Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe? - Answer✔✔-5' G
Proper GC content of primer - Answer✔✔-35-65%
Inhibitors of PCR - Answer✔✔-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol
Blue
Order to add to a PCR reaction: - Answer✔✔-Master mix > patient sample > positive control >
negative control
Pre Area - Answer✔✔-Positive Pressure
Page 3/33
, EMILLECT 2024/2025 ACADEMIC YEAR ©2024 EMILLECT. ALL RIGHTS RESERVED. FIRST PUBLISH OCTOBER 2024.
Post Area - Answer✔✔-Negative Pressure
Which PCR requires two steps for specificity? - Answer✔✔-Nested PCR
Which microorganism is detected by branched DNA amplification? - Answer✔✔-DNA or RNA
Which technique uses RNA, antibodies, and DNA? - Answer✔✔-Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation - Answer✔✔-Bisulfite
DNA
What tests are isothermal? - Answer✔✔-NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? - Answer✔✔-AML
T-cell gene rearrangement is seen in what malignancy? - Answer✔✔-Sezary Syndrome
Which translocation causes Acute Premyelocytic Leukemia? - Answer✔✔-t 15;17
Ewing Sarcoma - Answer✔✔-t 11;22
Synovial Sarcoma - Answer✔✔-t X;18
Follicular Lymphoma - Answer✔✔-t 14;18
What genes would be screened in a colorectal panel? - Answer✔✔-MSH2 & MLH1
What gene mutation causes a high risk of breast or ovarian cancer? - Answer✔✔-BRCA1 and
BRCA2
What does MSI test for? - Answer✔✔-Mutations in mismatch repair genes (MMR)
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