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CGMBS ch. 11 Detection and Identification of Microorganisms Questions and Answers 2024/2025 $11.49
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CGMBS ch. 11 Detection and Identification of Microorganisms Questions and Answers 2024/2025
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Salmonella typhi specimen collection → initially present in peripheral bloo but not in urine or stool util at least 2 weeks after infection Smallpox must be handled only in approved laboratories → level 4 containment When processing whole blood, why is it important to remove homoglobin �...

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  • October 15, 2024
  • 14
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • cgmbs ch 11 detection and identification of micr
  • salmonella typhi specimen collection initially p

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2 0 2 4 /2025 | © copyright | This work may not be copied for profit gain Excel!


CGMBS ch. 11 Detection and
Identification of Microorganisms
Questions and Answers 2024/2025
Salmonella typhi specimen collection


→ initially present in peripheral bloo but not in urine or stool util at least 2 weeks after
infection


Smallpox must be handled only in approved laboratories

→ level 4 containment


When processing whole blood, why is it important to remove homoglobin

→ can inhibit DNA polymerase and prevent amplification of nucleic acid resulting in
false-negative PCR result


Inhibitors or DNA pol. in urine


→ -nitrate
→ -crystals
→ -hemoglobin
→ -beta-human chorionic gonadotropin


amplification control

→ rule out false-negatives; detects target that is always present


Housekeeping genes in prokaryotes

→ groEL, rpoB, recA, gyrB


Housekeeping genes in eukaryotes

1|Page| GradeA+ | 2 0 0 2 5

, 2 0 2 4 /2025 | © copyright | This work may not be copied for profit gain Excel!

→ B-actin, glyceraldehyde-3-phosphate, interferon-y, extrinsic homologous control, human
mitochondril DNA, peptidylprolyl isomerase A


Homologous extrinsic control

→ target-derived control with a non-target-derived sequence insert; control is added to
every sample after nucleic acid extraction and before amplification


Heterologous extrinsic controls

→ non-target-derived controls that are added to every sample before nucleic acid
extraction; second set of primers must also be added to the reaction for the conrol to
be amplified


Heterologous intrinsic ontrols


→ nontarget sequences natrually present in the sample, such as eukaryotic genes in a test
for microorganisms


Reasons for false-negative results on a sample

→ 1. organism may be present, but nucleic acid was degraded during collection,
transport, and or extraction
→ 2. amplification procedures may be inhibited by substances present in the specimen


Sequencing of the DNA region encoding 16S rRNA is performed to determine


→ the evolutionary and genetic relatedness of microorganisms and has driven changes in
microorganism nomenclature


Microbiological identification and epidemiology

→ mass spectrometry of microbial proteins


Bordetella pertussis



1|Page| GradeA+ | 2 0 0 2 5

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