MCB 2610 Exam 1 Microscopy
numerical aperture - correct answer ✔ability of the lens to gather light -
depends on the property of the lens and the refractive index of the medium
refractive index - correct answer ✔how much a substance bends a light ray
immersion oil - correct answer ✔oil placed on a slide to minimize refraction
of the light entering the lens and increasing the numerical aperture and
resolution
working distance - correct answer ✔distance between the surface of lens
and the surface of cover glass or specimen when it is in sharp focus
resolving power - correct answer ✔ability to distinguish close objects as
separate - the most important property
bright field microscope - correct answer ✔- commonly used in microbiology
lab
- produces a dark image against brighter background
- can be used for both stained and unstained specimens
- has parfocal microscopes
parfocal (microscopes) - correct answer ✔remain in focus when objectives
are changed
dark field microscope - correct answer ✔- image is made by light reflect or
refracted by specimen
, - produces a bright image of the object against a dark background
- used to observe unstained preparations
- uses a hollow cone of light
phase contract microscope - correct answer ✔- coverts slight differences in
refractive index and cell density into easily detected variations in light intensity
- image is produced where cells are dark and the background is light
- uses a hollow cone of light
differential interference microscope - correct answer ✔- similar to phase
contract where the image detects differences in refractive index and thickness
of different part of specimen
- uses two beams of polarized light to create 3D image
- can observes unstained cells that appear bright and 3D
Fluorescence microscope - correct answer ✔- exposes specimen in
ultraviolet, violet, or blue light
- stained with fluorochromes
- shows bright image of the object from fluorescent light emitted by the
specimen
confocal microscope - correct answer ✔- creates sharp, composite 3D image
of specimens by using laser beam, aperture to eliminate stray light, and
computer interface
- usually fluorescently stained
light microscope - correct answer ✔- a type of optical instrument with lenses
that refract visible light to magnify images of specimens
- has a max magnification of 1500x with a resolving limit of 0.2 um
numerical aperture - correct answer ✔ability of the lens to gather light -
depends on the property of the lens and the refractive index of the medium
refractive index - correct answer ✔how much a substance bends a light ray
immersion oil - correct answer ✔oil placed on a slide to minimize refraction
of the light entering the lens and increasing the numerical aperture and
resolution
working distance - correct answer ✔distance between the surface of lens
and the surface of cover glass or specimen when it is in sharp focus
resolving power - correct answer ✔ability to distinguish close objects as
separate - the most important property
bright field microscope - correct answer ✔- commonly used in microbiology
lab
- produces a dark image against brighter background
- can be used for both stained and unstained specimens
- has parfocal microscopes
parfocal (microscopes) - correct answer ✔remain in focus when objectives
are changed
dark field microscope - correct answer ✔- image is made by light reflect or
refracted by specimen
, - produces a bright image of the object against a dark background
- used to observe unstained preparations
- uses a hollow cone of light
phase contract microscope - correct answer ✔- coverts slight differences in
refractive index and cell density into easily detected variations in light intensity
- image is produced where cells are dark and the background is light
- uses a hollow cone of light
differential interference microscope - correct answer ✔- similar to phase
contract where the image detects differences in refractive index and thickness
of different part of specimen
- uses two beams of polarized light to create 3D image
- can observes unstained cells that appear bright and 3D
Fluorescence microscope - correct answer ✔- exposes specimen in
ultraviolet, violet, or blue light
- stained with fluorochromes
- shows bright image of the object from fluorescent light emitted by the
specimen
confocal microscope - correct answer ✔- creates sharp, composite 3D image
of specimens by using laser beam, aperture to eliminate stray light, and
computer interface
- usually fluorescently stained
light microscope - correct answer ✔- a type of optical instrument with lenses
that refract visible light to magnify images of specimens
- has a max magnification of 1500x with a resolving limit of 0.2 um
