MCB 2610 Lab Exam 1
T streak method - correct answer ✔ussed to isolate colonies
1. steralize loop by placing in flame
2. dip into media
3. spread on zig zap in 1
4. steralize then drag from 1 to 2
5. steralize than drag from 2 to 3
colony - correct answer ✔community of genetically identical cells that can be
seen without a microscope
liquid vs solid media - correct answer ✔liquid= grow large amounts of
bacteria for testing ex. nutrient broth
solid= examine morphology, isolation ex. nutrient agar, solid polysacharide
complex vs defined media - correct answer ✔complex= don't know whats in
it, yeast extract, TSB broth less nutrients, general purpose media
defined= know whats in it ex. synthetic, has more nutrients
not all bacteria can be cultured
selective media vs differential - correct answer ✔suppress unwanted
microbes and encourage desired microbes ex. sf
differential= allows bacteria to be differentiated from others by color change
ex. macconkeys
, SF medium - correct answer ✔selective= e faecalis
grows E. faecalis= intestinal bacterium
contains sodium azide
inhibits gram negtive growth
ferments glucose to only grow gram +
control= macconkeys bc gram + can't grow
MacConkey Agar - correct answer ✔SELECTIVE and DIFFERENTIAL
selective= gram negative organism by bile salts, coliforms
differential = lactose (lactose positive organism will look pink negative will be
clear)
control= na glucose plate bc everything will grow on it
hydrolytic enzymes - correct answer ✔break down proteins and certain
carbohydrates.
amylase= breaks down starch
casease (casinase)= breaks down milk protein casein
gelatinase= breaks down gelatin
amylase test - correct answer ✔plate contains starch breaks down starch,
requires iodine which stains starch
positive= amber color
negative= black color
caseinase test - correct answer ✔medium contains casein
pos= clearing
negative= no clearing around growth'
T streak method - correct answer ✔ussed to isolate colonies
1. steralize loop by placing in flame
2. dip into media
3. spread on zig zap in 1
4. steralize then drag from 1 to 2
5. steralize than drag from 2 to 3
colony - correct answer ✔community of genetically identical cells that can be
seen without a microscope
liquid vs solid media - correct answer ✔liquid= grow large amounts of
bacteria for testing ex. nutrient broth
solid= examine morphology, isolation ex. nutrient agar, solid polysacharide
complex vs defined media - correct answer ✔complex= don't know whats in
it, yeast extract, TSB broth less nutrients, general purpose media
defined= know whats in it ex. synthetic, has more nutrients
not all bacteria can be cultured
selective media vs differential - correct answer ✔suppress unwanted
microbes and encourage desired microbes ex. sf
differential= allows bacteria to be differentiated from others by color change
ex. macconkeys
, SF medium - correct answer ✔selective= e faecalis
grows E. faecalis= intestinal bacterium
contains sodium azide
inhibits gram negtive growth
ferments glucose to only grow gram +
control= macconkeys bc gram + can't grow
MacConkey Agar - correct answer ✔SELECTIVE and DIFFERENTIAL
selective= gram negative organism by bile salts, coliforms
differential = lactose (lactose positive organism will look pink negative will be
clear)
control= na glucose plate bc everything will grow on it
hydrolytic enzymes - correct answer ✔break down proteins and certain
carbohydrates.
amylase= breaks down starch
casease (casinase)= breaks down milk protein casein
gelatinase= breaks down gelatin
amylase test - correct answer ✔plate contains starch breaks down starch,
requires iodine which stains starch
positive= amber color
negative= black color
caseinase test - correct answer ✔medium contains casein
pos= clearing
negative= no clearing around growth'
