BIOCHEM MIDTERM LAB EXAM. THE LATEST MIDTERM EXAM REVISION QUESTIONS AND CORRECT ANSWERS (GRADED A+) (100% GUARANTEE) (2024 UPDATE)
2 views 0 purchase
Course
Biochem
Institution
Biochem
Describe the DNA molecule; and identify the characteristics of DNA molecule that made it
possible for it to be isolated. - answer- learning outcomes for nucleic acid isolation and analysis
Reagent used in DNA analysis - answer- diphenylamine reagent
How many ml of diphenylamine reagent is used -...
Describe the DNA molecule; and identify the characteristics of DNA molecule that made it
possible for it to be isolated. - answer- learning outcomes for nucleic acid isolation and analysis
Reagent used in DNA analysis - answer- diphenylamine reagent
How many ml of diphenylamine reagent is used - answer- 2ml
How many minutes do you need to boil the samples - answer- 5 to 10 minutes
What is substituted in dna analysis - answer- 2 ml of 0.1 % solutions of ribose
Lysis method used - answer- chemical method
Solution used for the purpose of breaking open cells for use in molecular biology experiments that
analyze the labile macromolecules of the cells - answer- lysis buffer
It helps neutralize the DNA charge and make the molecule less hydrophilic, meaning it becomes
less soluble in water. It also helps to remove proteins that are bound to the DNA and to keep the
proteins dissolved in the water. - answer- salt solution
Precipitate DNA into a visible form. Also, it's used in DNA washing and storing. - answer-
alcohol
Soluble to water - answer- hydrophilic
,Insoluble to water - answer- hydrophobic
What part of DNA extraction do lysis method and lysis buffer belong? - answer- cell lysis
What part of dna extraction does salt solution and alcohol belong to? - answer- dna precipitation
What part of DNA extraction does distilled water belong? - answer- DNA purification
Color of DNA in diphenylamine test - answer- blue
Complete name of test in DNA analysis - answer- dische diphenylamine test
Color of RNA in diphenylamine test - answer- clear
Acidic conditions convert deoxyribose to a molecule that binds with diphenylamine to form a blue
complex. - answer- generalization of dische diphenylamine test
What are the lysis agents - answer- detergent
Purposes of DNA analysis - answer- genetic testing, body identification and analysis of forensic
evidence
What is the test used to gather cheek cells - answer- buccal swab
1. Collect skin cells
2. Bust cells open to release DNA
3. Separate DNA from protein debris
4. Isolate concentrated DNA - answer- steps for purifying DNA
, It disrupts the cell and nuclear envelope causing the cells the burst open - answer- detergent
It cuts the histones to free DNA - answer- proteinase k
Result for glucose in the dische diphenylamine test - answer- green
A linear double stranded polymer made up of ? - answer- deoxyribonucleotides
A very long thread like molecule made up of a very large number of deoxyribonucleotides joined
together. - answer- dna
It is made up of a sugar called deoxyribose, a nitrogenous base, and a phosphate group. - answer-
deoxyribonucleotide
Located in the nucleus - answer- dna
Nucleotides are joined together by a __________ in a condensation reaction. - answer-
phosphodiester bond
It is formed when two strands are joined together by hydrogen bonds. - answer- double helix
It is antiparallel because both the strands run in the opposite direction and are parallel to each other
- answer- double helix
It is a linear polymer in which nucleotides are linked together by means of phosphodiester bridges.
- answer- rna
What are three different types of rna - answer- mrna, trna, rrna
They usually have secondary structure, consisting of stem and loop domains. - answer- rna
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller pharismusyoka99. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.49. You're not tied to anything after your purchase.
