MCB*2050 Molecular Biology of the Cell W21 Midterm Examination, SAMPLE EXAM with Prof. K. Yankulov University of Guelph
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Course
MCB*2050 Molecular Biology of the Cell
Institution
MCB*2050 Molecular Biology Of The Cell
MCB*2050 Molecular Biology of the Cell W21 Midterm Examination, SAMPLE EXAM with Prof. K. Yankulov University of Guelph
PART A (Choose only one and the best answer among the choices given.)
1. What happens when you use a mixture of four fluorescent 2′,3′-dideoxyribonucleoside triphosphates ...
MCB*2050 Molecular Biology of the Cell W21 Midterm
Examination, SAMPLE EXAM with Prof. K. Yankulov
University of Guelph
MCB*2050 Molecular Biology of Cell
Midterm Examination, SAMPLE
EXAM
Instructor: Prof. K. Time: 90
Yankulov minutes
The midterm will consists of two parts: Part A and B.
Part A will consists of 20-40 multiple choice questions. 6-12 of these questions will be
derived from the online quizzes pools, 1-2 questions from each quiz. There will be
questions on the material from tutorials and from the lectures
PART B consists of 4-5 short answer questions. One of these questions will be from the
Tutorials. 3- 4 questions will be on the lecture material.
Page 1 of
, PART A (Choose only one and the best answer among the choices given.)
1. What happens when you use a mixture of four fluorescent 2′,3′-
dideoxyribonucleoside triphosphates in DNA sequencing protocols?
a. Multiple DNA chains that terminate at the 3’ ends at all possible
nucleotide positions are produced
b. When a 2′,3′-dideoxyribonucleoside triphosphate is added to the 5’ end
of a DNA chain, that chain can no longer be extended
c. The produced DNA chains will contain all four 2′,3′-
dideoxyribonucleoside triphosphates and these can be detected
by spectrophotometer
d. The incorporation of a fluoresent 2′,3′-dideoxyribonucleoside triphosphate
will intiate a chemical reaction that produces light in a well and can be
detected by a scanning camera
e. None of the above answers is correct
2. The regulation of gene expression through heritable modifications in
chromatin structure is called:
a. Epigenetic regulation
b. Signal transduction regulation
c. Transcriptional regulation
d. Gene expression regulation
e. Genetic regulation
3. In eukaryotic cells, gene regulation occurs at multiple levels.
Enhancer elements represent a component of which level of control?
a. Translational control
b. Post-translational control
c. Transcriptional control
d. Post-translational control
e. mRNA stability
4. Histone acetyl transferases (HATs) acetylate i) residues in ii) of
histones resulting in a loosening of the DNA-nucleosome interactions which
acts to
iii) transcription.
a. i) Lysine; ii) N-termini; iii) promote
b. i) Lysine; ii) core domain; iii) promote
c. i) Serine; ii) N-termini; iii) decrease
d. i) Serine; ii) core domain; iii) decrease
e) i) Lysine; ii) N-termini; iii) promote
5. The image below shows the results of a gel shift assay. Each column (i.-vi.)
contains a sample with the same radioactive oligonucleotide probe
complementary to a region of DNA which is suspected to be an enhancer
sequence. This DNA is known to bind several transcription factors of the same
family (HFN).
Page 2 of
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