DNA Biology & Technology: Gel Electrophoresis Questions and Answers Graded A+
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Course
DNA Biology & Technology: Gel Electrophoresis
Institution
DNA Biology & Technology: Gel Electrophoresis
DNA Biology & Technology: Gel
Electrophoresis Questions and Answers
Graded A+
What is the significance of using a DNA ladder in gel electrophoresis?
A DNA ladder serves as a size reference, allowing for the estimation of the sizes of unknown
DNA fragments by comparing them to known standar...
DNA Biology & Technology: Gel
Electrophoresis Questions and Answers
Graded A+
What is the significance of using a DNA ladder in gel electrophoresis?
✔✔A DNA ladder serves as a size reference, allowing for the estimation of the sizes of unknown
DNA fragments by comparing them to known standards.
How does the electric field impact the movement of DNA in gel electrophoresis?
✔✔The electric field causes negatively charged DNA molecules to migrate towards the positive
electrode, facilitating their separation based on size.
What happens if the gel is not properly polymerized before loading samples?
✔✔If the gel is not properly polymerized, it can result in inconsistent pore sizes, leading to poor
separation and distorted DNA bands.
Why is it important to visualize DNA bands immediately after gel electrophoresis?
✔✔Immediate visualization helps prevent the diffusion of bands, which can lead to blurring and
loss of resolution in the final image.
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, How can gel electrophoresis assist in forensic analysis?
✔✔Gel electrophoresis can separate DNA from crime scene samples, helping forensic scientists
match DNA profiles to suspects or victims.
What might indicate contamination in a gel electrophoresis experiment?
✔✔Unexpected bands or smears in the gel that do not correspond to the expected results can
suggest contamination from foreign DNA.
How does temperature affect the electrophoresis process?
✔✔Higher temperatures can increase the mobility of DNA but may also lead to the degradation
of samples, making it crucial to maintain optimal temperatures.
What role does ethidium bromide play in gel electrophoresis beyond staining?
✔✔Ethidium bromide not only stains the DNA for visualization but also helps in assessing the
integrity of the DNA by highlighting intact versus degraded fragments.
What are the advantages of using pulsed-field gel electrophoresis (PFGE) over standard gel
electrophoresis?
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