Gel Electrophoresis Questions and Answers Already Passed
2 views 0 purchase
Course
Gel Electrophoresis
Institution
Gel Electrophoresis
Gel Electrophoresis Questions and
Answers Already Passed
What type of gel is typically used for separating nucleic acids?
Agarose gel is typically used for separating nucleic acids like DNA and RNA.
How does the size of DNA fragments influence their migration rate in gel electrophoresis?
...
Gel Electrophoresis Questions and
Answers Already Passed
What type of gel is typically used for separating nucleic acids?
✔✔Agarose gel is typically used for separating nucleic acids like DNA and RNA.
How does the size of DNA fragments influence their migration rate in gel electrophoresis?
✔✔Smaller DNA fragments migrate faster through the gel than larger fragments due to less
resistance in the gel matrix.
What is the purpose of applying a dye to the gel before electrophoresis?
✔✔Dyes are used to track the progress of the electrophoresis run and visualize the migration of
the samples.
What kind of current is applied during gel electrophoresis?
✔✔A direct current (DC) is applied during gel electrophoresis to drive the movement of charged
molecules through the gel.
Why is it important to allow the gel to solidify properly before use?
1
, ✔✔Proper solidification ensures that the gel matrix maintains its structure, allowing for accurate
separation of the samples.
What is a potential hazard when using ethidium bromide for staining DNA?
✔✔Ethidium bromide is a mutagen and can intercalate into DNA, posing a potential health
hazard if not handled properly.
How can you determine the success of a restriction enzyme digestion using gel electrophoresis?
✔✔By comparing the band pattern of the digested DNA to a ladder, you can assess whether the
digestion occurred as expected.
What effect does voltage have on the resolution of DNA fragments during electrophoresis?
✔✔Higher voltage can speed up the run but may reduce resolution, while lower voltage can
improve resolution but take longer to separate fragments.
Why is it necessary to include a control sample when running gel electrophoresis?
✔✔Including a control sample helps validate the results by providing a standard for comparison
and confirming that the gel worked properly.
2
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller BrilliantScores. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
