DNA Gel Electrophoresis Exam Questions and Answers Latest Update Graded A+
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DNA Gel Electrophoresis
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DNA Gel Electrophoresis
DNA Gel Electrophoresis Exam Questions and Answers Latest Update Graded A+
How does the process of gel electrophoresis separate DNA fragments? - Answers It uses an electric current to separate different sized molecules of DNA in a porous sponge-like matrix.
What is the purpose of the agarose gel?...
DNA Gel Electrophoresis Exam Questions and Answers Latest Update Graded A+
How does the process of gel electrophoresis separate DNA fragments? - Answers It uses an electric
current to separate different sized molecules of DNA in a porous sponge-like matrix.
What is the purpose of the agarose gel? - Answers To separate the different sized fragments of DNA.
3. What is the purpose of adding blue "tracking" dye to the DNA samples? - Answers It makes it easier to
load the samples and visually track the migration of DNA through the gel.
4. Explain why DNA has an overall negative charge. - Answers Phosphate groups in the DNA backbone
carry negatively-charged oxygen molecules giving the phosphate-sugar backbone of DNA an overall
negative charge.
5. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? -
Answers The negatively charged DNA can be pulled toward the positive field of the gel.
6. Explain how an agarose gel can separate DNA fragments of different lengths. - Answers Smaller
fragments move faster, and therefore further, than larger fragments as they snake through the gel.
7. What is the purpose of ethidium bromide? - Answers Ethidium bromide is a dye used by scientists to
see where the DNA fragments are located in the gel.:It binds to the DNA and glows when illuminated
with UV light.
8. Why is a marker used when running the fragments through the gel? - Answers A marker contains DNA
fragments of known size. Markers are run in every gel for comparison with the unknown fragments in
other gel lanes.
9. What is a restriction map? - Answers It shows where restriction enzymes cut the original piece of
DNA.
Building blocks of DNA are called: - Answers Nucleotides
What part of DNA gives the molecule a negative charge? - Answers Phosphate
Which direction will the DNA travel towards? - Answers Towards the + end
What do restriction enzymes do? - Answers Cut the DNA
You want to use different restriction enzymes to cut the DNA of the guppies so that you can compare
them accurately. - Answers False
What fragments of DNA travel fastest through the gel? - Answers small
Where does transcription n occur? - Answers in the nucleus
Ribosomes - Answers don't have a cell membrane
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