SDS gel electrophoresis Review Questions and Answers Graded A+
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Course
SDS gel electrophoresis
Institution
SDS Gel Electrophoresis
SDS gel electrophoresis Review Questions and Answers Graded A+
Mobility - Answers term that describes how easily the ions move in an electric field
electrophoresis - Answers process by which charged molecules are separated in an electric field due to their differential mobilities
mobility factor...
have a carefully controlled and adjustable porosity
What is the importance of pore size? - Answers it is a major contributor to the frictional coefficient f
SDS page sieving effect-- - Answers the sieving effect is the principle means of fractionation
What does the sieving effect permit? - Answers the separation of molecules of identical charge/mass
ratios but of different sizes
Gel filtration difference - Answers gel filtration- molecules are excluded from the interior of the gel
beads by their size so that the largest molecules elute first
SDS page difference - Answers gel matrix like substance rather than a bead, acts like a sieve, where
frictional forces decrease the mobility of larger molecules more than those of small
What is the supporting medium of choice? - Answers polymerized acrylamide
What is the cross linking agent of choice? - Answers Bis
Average size of the pores can be controlled by..... - Answers varying the amount of monomer used
varying the degree of cross linking
5-12 - Answers 20-150 thousand
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