Measures and detects variations in electrical resistance between two electrodes as cells traverse a
sensing aperture.
**How is cell population assessed in an automated cell counter?**
Cell population assessment is based on cell size.
**Light Scatter**
Blood is hydrodynamically focused using a sheathing fluid that propels cells toward the center of
the column. As the laser beam is interrupted, cells are counted and sized.
1
, **In WBC Histograms, what does 35-90 fL represent?**
Lymphocytes
**In WBC Histograms, what does 90-160 fL represent?**
Mononuclear Cells: This includes Monocytes, Blasts, Immature Granulocytes, and reactive
lymphocytes.
**In WBC Histograms, what does 160-450 fL represent?**
Granulocytes
**In a RBC Histogram, what volume is classified as RBCs? (in fL)**
Greater than 36 fL
**In a Platelet histogram, what is the range for counted particles?**
2-20 fL
**What is the volume drawn by an automated counter?**
100 microliters
**Which two components are included in the RBC bath dilution of an automated counter?**
The RBCs and Platelets
**Which two components are included in the WBC bath dilution of an automated counter?**
WBCs and Hemoglobin
**Directly Measured Parameters of WBCs**
Impedance
Counts all cells greater than 35 fL
2
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