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Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+ $7.99   Add to cart

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Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+

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Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+ plasmid/vector - Answers small circular piece of DNA found in bacteria, often carry antibiotic resistance restriction enzymes - Answers molecular scissors restriction endonucleases - Answers act on DNA. reco...

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  • October 21, 2024
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  • Recombinant DNA Technology Chapter 7
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Recombinant DNA Technology Chapter 7 Review Questions with Complete Solutions Graded A+

plasmid/vector - Answers small circular piece of DNA found in bacteria, often carry antibiotic resistance

restriction enzymes - Answers molecular scissors

restriction endonucleases - Answers act on DNA. recognize specific sequences and cut them. involved in
"restricting" growth of bacteriophage in certain bacteria

cloning - Answers fusing of 2 pieces of DNA together

recombinant DNA molecules - Answers constructed with DNA from different species. these can be found
in nature. (e.g., bacteriophage infecting bacteria and inserting its DNA into host)

methylation

adenine - Answers bacterial genome protects itself from EcoR1 by ______ at specific _____ residues

gel electrophoresis

agarose (or polyacrylamide) - Answers restriction fragments can be separated by __________ in
_______ gels

ethidium bromide - Answers the separated fragments of gel electrophoresis can be visualized by staining
with _________

DNA ligase

recombinant DNA - Answers the fragmented DNA and a vector are combined by ______ which
generates ______

plasmids

bacteriophages

viruses

artificial chromosomes - Answers cloning vectors can be:

MCS (multiple cloning site) - Answers multiple unique sites close together that can by cut by restriction
enzymes

ori - Answers origin of replication is abbreviated

Kan^r - Answers antibiotic resistance genes are abbreviated

rop - Answers gene that regulates replication for ~20 copies of the plasmid per cell (how many to
replicate for each cell)

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