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DNA Sequencing and Electrophoresis Techniques Exam Questions and Answers Graded A+ $7.99   Add to cart

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DNA Sequencing and Electrophoresis Techniques Exam Questions and Answers Graded A+

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DNA Sequencing and Electrophoresis Techniques Exam Questions and Answers Graded A+ Agarose Gel Electrophoresis - Answers Analyzes DNA fragments from 100 bp to 20,000 bp. Pulsed Field Gel Electrophoresis (PFGE) - Answers Separates DNA fragments larger than 20,000 bp. Electrophoresis Duration - An...

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  • October 21, 2024
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  • DNA Sequencing and Electrophoresis Techniques
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DNA Sequencing and Electrophoresis Techniques Exam Questions and Answers Graded A+

Agarose Gel Electrophoresis - Answers Analyzes DNA fragments from 100 bp to 20,000 bp.

Pulsed Field Gel Electrophoresis (PFGE) - Answers Separates DNA fragments larger than 20,000 bp.

Electrophoresis Duration - Answers Typically lasts 18 to 24 hours for PFGE.

Polyacrylamide Gel Electrophoresis - Answers Separates polynucleotides from 10 to 4000 bases.

Acrylamide Gel Composition - Answers Made from acrylamide, bis-acrylamide, urea, buffer.

Electrophoresis Voltage - Answers Applied potential difference around 1500 volts.

Denatured DNA - Answers Single-stranded DNA maintained by heat and urea.

Ethidium Bromide Staining - Answers Used to visualize DNA bands in gels.

Autoradiography - Answers Detects radioactive DNA via X-ray film exposure.

Dideoxy Chain Termination Method - Answers Automated DNA sequencing method developed by
Sanger.

Template DNA - Answers Single-stranded DNA used for sequencing reactions.

Oligonucleotide Primer - Answers Short DNA sequence that anneals to template.

DideoxyNTPs - Answers Terminate DNA chain growth due to missing hydroxyl.

Radioactively Labeled dNTPs - Answers Contain 32P or 35S for detection in sequencing.

High-Resolution Polyacrylamide Gel - Answers Separates polynucleotide chains differing by one
nucleotide.

Fluorescent Dyes in Sequencing - Answers Different colors for each dideoxyNTP in automation.

Contig - Answers Long contiguous sequence compiled from overlapping fragments.

Open Reading Frames (ORFs) - Answers Sequences starting with start codon and ending with stop.

Genome Sequencing - Answers Entire bacterial genomes sequenced within months.

Next-Generation Sequencing - Answers Supersedes dideoxy method for genome sequencing.

Heat Distribution in Electrophoresis - Answers Aluminium plates prevent glass cracking from heat.

X-ray Film Development - Answers Reveals bands from radioactive DNA on film.

Robotic Template Preparation - Answers Automates sequencing process for efficiency.

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