DNA SEQUENCING AND MICROARRAY EXAM QUESTIONS AND ANSWERS GRADED A+
8 views 0 purchase
Course
DNA SEQUENCING AND MICROARRAY
Institution
DNA SEQUENCING AND MICROARRAY
DNA SEQUENCING AND MICROARRAY EXAM QUESTIONS AND ANSWERS GRADED A+
DNA SQUENCING - Answers It determines the exact sequence of nucleotides/ bases in a DNA molecule
ACTG (nucleotides) - Answers Encode biological informations
sequence of DNA - Answers establishing the__________ is key to understan...
sequence of DNA - Answers establishing the__________ is key to understanding the functions of genes
and other parts of the genome
-detection of mutations
-typing microorganisms
-identifying human haplotyles
-designating polymorphisms - Answers Uses of DNA Sequencing
Direct determination of nucleotide sequence or DNA sequencing - Answers Most effective molecular
method to identify genetic lesions (mutations)
-Maxam-Gilbert Method
-Sanger Method - Answers Manual Sequencing Methods (used most extensively)
Allan M. Maxam and Walter Gilbert (Late 1970s) - Answers Maxam-Gilbert Method developer
dsDNA or ssDNA version of DNA region; with one end radioactive labeled (32p) - Answers MAXAM-
GILBERT is a chemical sequencing that requires:
MG Method template - Answers Aliquoted into four (4) tubes; each was treated w diff chemical
with/without high salt
-Dimethylsulphate
-Formic Acid
-Hydrazine
-Hydrazine + salt - Answers Specific base reactions (MGS) base modifier
10% piperidine - Answers Strong reducing reagent that causes ssDNA to break at specific nucleotides
upon addition
Dimethylsulphate; GUANINE (chain) - Answers breaks the chain of _________ by methylating G at 25
degrees celsius for 4 minutes
Formic Acid; Guanine + Adenine breaks - Answers breaks the chain of _______ by protonating purines at
25 degrees celsius for 5 minutes
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller TutorJosh. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.29. You're not tied to anything after your purchase.
