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Advancements in DNA Sequencing Technologies Exam Questions and Answers Latest Update

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Advancements in DNA Sequencing Technologies Exam Questions and Answers Latest Update Sanger Sequencing - Answers First generation DNA sequencing method developed by Frederick Sanger. Next Generation Sequencing (NGS) - Answers Advanced sequencing technologies allowing massive parallel sequencing....

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  • October 21, 2024
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Advancements in DNA Sequencing Technologies Exam Questions and Answers Latest Update

Sanger Sequencing - Answers First generation DNA sequencing method developed by Frederick Sanger.

Next Generation Sequencing (NGS) - Answers Advanced sequencing technologies allowing massive
parallel sequencing.

Chain-Termination Method - Answers Technique using ddNTPs to stop DNA strand extension.

Phi X 174 - Answers First genome sequenced using Sanger method in 1977.

Dideoxyribonucleotide (ddNTP) - Answers Modified nucleotide lacking 3'-OH group for termination.

Capillary Electrophoresis - Answers Technique for separating DNA fragments by size.

Fluorescent Dye - Answers Label used to detect DNA fragments in sequencing.

Human Genome Project (HGP) - Answers International project to sequence and map human genes.

Sequencing Read Length - Answers Typical length of sequenced DNA, 750-800 bases.

Illumina Sequencing - Answers Dominant NGS technology known for high throughput.

Library Preparation - Answers Process of preparing DNA/RNA samples for sequencing.

Bridge Amplification - Answers Technique to amplify DNA fragments on a flow cell.

Sequencing by Synthesis (SBS) - Answers Method where nucleotides are added one at a time.

PCR Amplification - Answers Process to increase quantity of DNA fragments.

Flow Cell - Answers Surface where DNA clusters are formed and sequenced.

Base Calling - Answers Determining DNA sequence from digital fluorescence data.

Data Analysis - Answers Interpreting sequencing data to extract meaningful information.

Massively Parallel Sequencing - Answers Simultaneous sequencing of numerous DNA samples.

Cost of HGP - Answers Approximately US$3 billion for sequencing human genome.

Sequencing Adaptors - Answers Short DNA sequences attached to fragments for sequencing.

Image Processing - Answers Converting fluorescence signals into digital sequencing data.

Terminator Group - Answers Chemical group preventing further DNA extension during SBS.

Sequencing Applications - Answers Uses include whole genome, exome, and RNA sequencing.

Run Time - Answers Maximum run time of 2 hours for sequencing.

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