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CHEMISTRY – HEME DERIVATES EXAM QUESTIONS AND ANSWERS
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CHEMISTRY – HEME DERIVATES EXAMQUESTIONS AND ANSWERS
d
principle of the test reaction - ANSWER The principle of the occult blood
test depends upon the:
a. coagulase ability of blood
b. oxidative power of atmospheric oxygen
c. hydrogen peroxide in haemoglobin
d. peroxidase-like activity of haemoglobin
b
hemoglobin metabolism - ANSWER A breakdown product of hemoglobin
is:
a. lipoprotein
b. bilirubin
c. hematoxylin
d. bence-jones protein
a
Comparative mobilities due to structures of Hgb variants - ANSWER
Hemoglobin S can be separated from hemoglobin D by:
a. electrophoresis on a different medium and acidic pH
b. hemoglobin A2 quantitation
c. electrophoresis at higher voltage
d. Kleihauer-Betke acid elution
c
, Comparative mobilities due to structures of Hgb variants - ANSWER On
electrophoresis at alkaline pH, which of the following is the slowest
migrating hemoglobin?
a. Hgb A
b. Hgb S
c. Hgb C
d. Hgb F
d
Physical-chemical property of Hgb F - ANSWER The hemoglobin that is
resistant to alkali (KOH) denaturation is?
a. A
b. A2
c. C
d. F
c
Light-exposed bilirubin is oxidized (structurally altered). - ANSWER The
following bilirubin results are obtained on a patient:
day 1: 4.3 mg/dl (73.5 umol/l)
day 2: 4.6 mg/dl (78.7 umol/l)
day 3: 4.5 mg/dl (77.0 umol/l)
day 4: 2.2 mg/dl (37.6 umol/l)
day 5: 4.4 mg/dl (75.2 umol/l)
day 6: 4.5 mg/dl (77.0 umol/l)
Given that the controls were within range each day, what is a probable
explanation for the result on day 4?
a. no explanation necessary
b. serum, not plasma, was used for testing
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