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MCB 101 FINAL EXAM NEWEST 2024 ACTUAL EXAM QUESTIONS AND CORRECT ANSWERS | A+ GRADE STUDYGUIDE
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MCB 101 FINAL EXAM NEWEST 2024 ACTUAL EXAM QUESTIONS AND CORRECT ANSWERS | A+ GRADE STUDYGUIDE
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MCB 101 FINAL EXAM NEWEST 2024 ACTUAL EXAMQUESTIONS AND CORRECT ANSWERS | A+ GRADE
STUDYGUIDE
After you set up your antibiotic dilution series for determining the MIC of tertracycline,
what concentration of tetracycline should be present in tube #9 (in ug/mL) if you followed
the directions correctly? - ANSWER0.3900
Which tube in the antibiotic dilution series for determining the MIC of tetracycline
contains NO antibiotic - ANSWERtube 14
How much culture will you use for your spread plates to determine the MCB of
tetracycline? - ANSWER1000 uL
How many Luria plates will you use to determine the MCB of tetracycline in E. coli? -
ANSWERone plate for each MIC tube that did not have growth
How many bacteria will you inoculate on to each antibiotic containing plate as part of the
antibiotic susceptibility exercise? - ANSWER4
Which characteristic of an antibiotic is most useful in determining the appropriate
dosage to administer to a severely immunocompromised patient who currently has
Pneumocystis jirovecii pneumonia - ANSWERthe therapeutic index as this determines an
effective and safe dosage
Match the correct antibiotic with each statement:
interacts with the 30S ribosomal subunit - ANSWERtetracycline
Match the correct antibiotic with each statement:
inhibits protein synthesis - ANSWERtetracycline
Match the correct antibiotic with each statement:
interacts with the subunits of peptidoglycan directly - ANSWERvancomycin
Match the correct antibiotic with each statement:
can result in mottled colored teeth in children - ANSWERtetracycline
What is the term for the ability of a substance to inhibit an infection without harming the
host? - ANSWERselective toxicity
, What is the main distinction between antibiotics and disinfectants? - ANSWERantibiotics
are safe for internal use
Why is it important that an antibiotic used therapeutically have a narrow spectrum? -
ANSWERthey minimize the disruption of normal microflora in the patient
Many of the characteristics that make "good" antibiotics also apply to anti-cancer drugs.
While studying a potential novel anti-cancer drug, you apply the novel agent to purified
lung cancer cells in a cell culture experiment and determine the proportion of cell death
after a set period of time.After several trials of applying the agent only to purified cancer
cells, you determine that the agent significantly reduces the viability of the cancer cells
after 24 hours of treatment. Based on these data you triumphantly declare at the next
department conference that you have discovered a novel anti-cancer drug. Why is your
conclusion highly questionable? - ANSWERlack of controls
After several trials of applying the agent only to purified cancer cells, you determine that
the agent significantly reduces the viability of the cancer cells after 24 hours of
treatment. Based on these data you triumphantly declare at the next department
conference that you have discovered a novel anti-cancer drug.Your supervisor suggests
testing the agent again but including non-cancer cells. You find that the cancer cells as
well as the non-cancer cells have reduced viability. Which characteristic of a "good" drug
is broken by this finding? - ANSWERselective toxicity
Why must you centrifuge the donor strain, discard the media it was originally grown in,
and resuspend it in fresh Luria broth prior to mixing it with the recipient strain? -
ANSWERthe donor strain is grown in media containing the antibiotic kanamycin which
would inhibit the growth of the recipient if not removed
What volume of mating mix (mixture of donor and recipient) will you spread plate on to a
Luria Agar + DAP plate? - ANSWER200 ul
How will you harvest the mating mix (mixture of donor and recipient) after it has been
incubated? - ANSWERpouring sterile Luria broth on the plate and creating a slurry using
your plate spreader
On which type of media will only successfully transformed recipient cells grow? -
ANSWERLuria Agar + Kanamycin
How many control plates will you start on day 1 to evaluate the characteristics of the
donor and recipient strains? - ANSWER4
For each statement, indicate if it describes conjugation, transduction, or transformation:
utilizes free DNA in the environment - ANSWERtransformation
For each statement, indicate if it describes conjugation, transduction, or transformation:
requires the use of defective viral particles - ANSWERtransduction
For each statement, indicate if it describes conjugation, transduction, or transformation:
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