Exam (elaborations)

ASU Cell BIO 353 Exam 3 SA Practice Questions and Answers

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ASU Cell BIO 353 Exam 3 SA Practice Questions and Answers How do mitochondria get their lipids? Give a detailed answer. - Ans:-a. Lipids synthesized in ER b. transported to mitochondria c. switch of lipid takes place between ER and mitochondria & between mitochondrial membranes d. Membrane-tet...

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ASU Cell BIO 353 Exam 3 SA Practice
Questions and Answers

How do mitochondria get their lipids? Give a detailed answer. - Ans:✔✔-a. Lipids synthesized in ER

b. transported to mitochondria

c. switch of lipid takes place between ER and mitochondria & between mitochondrial membranes

d. Membrane-tethering & lipid transfer proteins in mitochondria provides FIRST insight into mechanisms

and transport processes

e. Mitochondria DO NOT participate in vesicle trafficking, obtain lipids from ER through mitochondrial-

associated ER membranes

List the chaperones in the order that they are used in the long path of getting a protein into the

mitochondrial matrix. (Note you should start in the cytoplasm.) - Ans:✔✔-a. cytoHSP70- in cytoplasm,

chaperone that coats mRNA

b. mitoHSP70- in between inner&outer mitochondrial membranes

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c. mitoHSP60- mitochondrial matrix, enables whole protein to come in w/ COOH end, uses ATP for

chaperone

Describe evolution of endomembrane system -include why it is important. Contrast this with the

evolution of the endosymbiotic origin of mitochondria and chloroplasts. - Ans:✔✔-a. endomembrane

system is involved in a comprehensive communication system between cells.

b. Components of endomembrane system are central to eukaryotic cell life

c. Endosymbiosis.

Analyze the signal sequence, and related characteristics that suggest that mitochondria and chloroplasts

did not originate as part of the evolution of the endomembrane system - Ans:✔✔-a. Components of

endomembrane system are central to eukaryotic cell life

b. Endosymbiosis- improved fitness of the cell

c. Mitochondria can make their own protein from circular DNA, now most has been outsourced to

nucleus with genes transcribed in cytoplasm

d. Signal sequence recruits HSP70 and coats growing protein

Explain how cells take in water and general food at the periphery of the cells. - Ans:✔✔-a. Food-

phagocytosis - plasma membrane is pushed out around food, creating two lamellipodia and fuse

together to form vesicle around food

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b. Water- pinocytosis - invagination forms around water, taken into cytoplasm through membrane

channels to avoid damaging cell if vesicle explodes- membrane "Straw" used to "sip" water

Explain how general food and food taken in by receptor mediated endocytosis merge with elements of

the endomembrane system to digest and absorb those nutrients (including cholesterol). - Ans:✔✔-a.

When a ligand binds to a receptor, receptor undergoes a conformational change.

b. Exposes binding site for adaptor protein AP-2

c. Capping occurs when same type of receptors bind to their ligands all over surface of cell come

together over small area; looks like baseball cap without the brim.

d. Adapter protein recruits triskelon to form clathrin coat

e. Receptor and ligand move using molecular motors

f. Amino acid subunits pumped into cytoplasm & indigestible components removed by exocytosis

Explain how membrane flow participates with membranes that are part of the endomembrane system,

but also part of the plasma membrane. - Ans:✔✔-a. Vesicular transport moves newly synthesized lipids,

transmembrane proteins, and luminal proteins within the endomembrane system.

b. Forms spherical vesicle

c. Vesicle is transported on microtubule pathway from ER to cis-face GA then move to cell periphery,

transfer to actin filament, and move to PM

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Exam (elaborations)

ASU Cell BIO 353 Exam 3 SA Practice Questions and Answers

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