BIO 353 Exam 1 Study Guide with Complete Solutions
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Course
BIO 353
Institution
BIO 353
BIO 353 Exam 1 Study Guide with
Complete Solutions
The Cell - Ans:-Basic unit of life
What are the major organelles/structures of a basic eukaryotic animal cell? - Ans:-Nucleolus,
nucleus, nuclear envelope, nuclear pore, chromatin (DNA), mitochondria, endoplasmic reticulum, golgi
complex, acti...
Describe optical tweezers and how they can be used. - Ans:✔✔-Instrument that uses a focused laser
beam to provide an attractive or repulsive force, depending on the refractive index mismatch to
physically hold and move microscopic objects
Can the cells be alive when using phase contrast? - Ans:✔✔-Yes
Describe the basic idea of epifluorescence microscopy - Ans:✔✔-The use of a fluorescence microscope
allows detection of specific proteins or other molecules/ions/organelles that are made fluorescent by
coupling them to a fluorochrome marker
Explain the steps involved in Indirect Immuno-fluorescence Microscopy - Ans:✔✔-1. Cells/tissues are
fixed (killed)
2. Exposed to the primary antibody, the antibody binds only to a specific protein it recognizes
3. Exposed to secondary antibody, the antibody binds only to the primary antibody. The secondary
antibody is coupled to a fluorochrome marker; thus location of protein in cells/tissues are fluorescently
labeled and may be viewed w/ a fluorescence microscope
Are the cells alive or dead for Indirect Immuno-fluorescence microscopy. - Ans:✔✔-Dead
What is GFP and how is it coupled to the proteins in different cellular structures? - Ans:✔✔-Green
Fluorescent Protein; it is inserted into the protein
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