BIO 353 Study Guide exam 1 with Complete Solutions
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Course
BIO 353
Institution
BIO 353
BIO 353 Study Guide exam 1 with
Complete Solutions
The cell - Ans:-The basic unit of structure and function in living things
What are the major Organelles/ structures of a basic eukaryotic cell? - Ans:-nucleus
nucleolus
ribosomes
smooth ER
rough ER
mitochondria
What are the 3 major discipl...
ii. exposed to primary antibody, antibody binds to specific protein (ie antigen)
iii. exposed to secondary antibody that is coupled to a flourochrome marker
What is GFP and how is it coupled to the proteins in different cellular structures (direct method)? -
Ans:✔✔-Green Fluorescent Protein binds the flourescent sntibody to the corresponding antigen on the
target protein
Whats the advantage of using GFP? - Ans:✔✔-cells are ALIVE
What are vital fluorescent dyes (direct method)? - Ans:✔✔-harmless dyes used to stain living tissue for
observation
confocal microscopy - Ans:✔✔-a light microscope that uses fluorescent stains and laser to make two-
and three-dimensional images
Advantages of confocal microscopy - Ans:✔✔-increased clarity of images and resolution
What is electron microscopy? - Ans:✔✔-technique used for obtaining high resolution images of
specimens
transmission electron microscope - Ans:✔✔-used to study the internal structure of cells
Advantage of electron microscope - Ans:✔✔-shorter wavelength of electron beam= increased resolution
and magnification
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