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BIOL 202 Week 6 Lab 6; Quantitation of Cultured Microorganisms 2024/2025 $15.99   Add to cart
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BIOL 202 Week 6 Lab 6; Quantitation of Cultured Microorganisms 2024/2025
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  • BIOL202 (BIOL202)
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  • Abacus College, Oxford

BIOL 202 Week 6 Lab 6; Quantitation of Cultured Microorganisms 2024/2025/BIOL 202 Week 6 Lab 6; Quantitation of Cultured Microorganisms 2024/2025

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  • October 23, 2024
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  • 2024/2025
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  • biol 202 week 6 lab 6
  • week 6 lab 6 quantitation of cultured microo

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  • Abacus College, Oxford
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  • BIOL202 (BIOL202)
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Quantitation of Cultured Microorganisms

Lab 6 Quantitation of Cultured Microorganisms - 100 points

Student Name: ………….
Student ID: ………
Course: BIOL202
✓ -Each question on the lab worksheet must be answered completely,
thoroughly, in complete sentences and correctly in order to be
considered for full credit
✓ -If the question asks you to do research or find a source, a reputable,
credible and/or scholarly source citation must be included in order to
be considered for full credit
✓ -If a math formula is required to arrive to an answer, work must be
shown otherwise, no credit will be awarded



Pre-Lab Questions
1. Question: A serial dilution of a sample has an unknown
concentration of microorganisms. A countable plate has 44
CFU. It was inoculated with 0.1 mL of a 10-1 dilution. What was
the population density of the original sample per milliliter? (10
points)


10−
- 44 1
x (calculate the power to simplify)
0.1ml
10
- 44 (multiply the numerator and denominator)
x 0.1ml
10 0
(simplify into single fraction)
- 44 ml
x

-100 44 x
= 4,400 CFU/ml
ml

2. Question: Compare and contrast the plate count methods for
bacterial and viral populations. (10 points)

`
Bacteria is identified through direct plate counts by colony forming unit (CFU). This
allows microbiologist to calculate colonies formed and they utilize the formula
CFU original CFU counted x dilution factor

, =
ml volume of culture plate(ml )
Viruses however cannot be traced on agar plates they must be identified utilizing plaque
assays. The virus must be diluted before obtaining a plate between 30 and 300 plaques
and utilize the plaque forming unit (PFU) . Both CFU and PFU share the same dilution

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