CURRENTLY TESTING SOLUTIONS OF ASU MIC 205 PENTON EXAM 1 QUESTIONS WITH ACTUAL CORRECT DETAILED ANSWERS
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Course
ASU MIC 205 PENTON
Institution
ASU MIC 205 PENTON
CURRENTLY TESTING SOLUTIONS OF ASU MIC 205 PENTON
EXAM 1 QUESTIONS WITH ACTUAL CORRECT DETAILED
ANSWERSCURRENTLY TESTING SOLUTIONS OF ASU MIC 205 PENTON
EXAM 1 QUESTIONS WITH ACTUAL CORRECT DETAILED
ANSWERSCURRENTLY TESTING SOLUTIONS OF ASU MIC 205 PENTON
EXAM 1 QUESTIONS WITH ACTUAL CORR...
CURRENTLY TESTING SOLUTIONS OF ASU MIC 205 PENTON
EXAM 1 QUESTIONS WITH ACTUAL CORRECT DETAILED
ANSWERS
What is a simple stain used for?
To increase contrast and visualize the basic shape and arrangement of cells.
How does a simple stain work?
By applying a single dye (like crystal violet or methylene blue) to the bacterial smear, causing all cells to
take on the same color.
What is a limitation of simple staining?
It does not distinguish between different types of cells.
What is a common example of a type of stain used in the differential staining technique?
Gram stain
What does the Gram stain differentiate between?
Gram-positive and Gram-negative bacteria
What is the purpose of a differential stain?
To differentiate between different types of bacteria or cellular structures based on their characteristics
How do cells appear differently after undergoing a differential stain?
Cells will take on different colors based on their properties, allowing for identification and classification
what does a gram stain detect
detects the pretense of bacteria and helps to differentiate between gram + and gram - based on cell wall
structure
,How does a gran stain differentiate bacterial cells
they get dyed a different color
both will be purple at first then they add iodine allowing color to stick to cell
then add alcohol or acetone which shows what they will be
what color is a gram positive bacteria
The thick peptidoglycan layer retains the crystal violet-iodine complex, so they remain purple.
what color is gram negative bacteria
pink, they don't retain the color so they counterstain with safranin and it absorbs the pink color.
What is the cell wall structure of Gram-positive bacteria?
Thick Peptidoglycan Layer
What component of Gram-positive bacteria's cell wall is involved in maintenance and protection?
Teichoic Acids
How do Gram-positive bacteria appear after the Gram staining process?
Purple under a microscope
Are Gram-positive bacteria generally more or less susceptible to antibiotics that target the peptidoglycan
layer?
More susceptible
What type of toxins do Gram-positive bacteria typically produce?
Exotoxins
Name two examples of Gram-positive bacteria.
Staphylococcus aureus, Streptococcus pneumoniae
What are the components of the cell wall structure in Gram-negative bacteria?
Thin Peptidoglycan Layer, Outer Membrane
How do Gram-negative bacteria stain in the Gram staining process?
Do not retain crystal violet, appear pink after counterstaining with safranin
Why are Gram-negative bacteria often more resistant to antibiotics?
Due to protective outer membrane, efflux pumps, and porins that limit drug entry
What can act as endotoxins in Gram-negative bacteria?
Lipopolysaccharides in the outer membrane
Provide examples of Gram-negative bacteria.
, Escherichia coli, Salmonella
What do penicillin antibiotics act on with the bacterial cell?
the peptidoglycan layer
Are gram + or gram -bacteria more susceptible to these antibiotics?
gram + are more susceptible due to their thick peptidoglycan layer. Gram - is less susceptible because it
has an outer membrane and a thin peptidoglycan layer making it harder for penicillin to
why doesn't penicillin harm eukaryotes?
Eukaryotic cells, such as human cells, do not possess peptidoglycan in their cell walls. Instead, they have
a different cellular structure, primarily consisting of a cell membrane. Since penicillin specifically targets
the bacterial cell wall, it does not affect eukaryotic cells, making it safe for use as an antibiotic in humans
and other eukaryotes.
What does an acid-fast stains
differentiate cells with a waxy cell wall that contains high amounts of mycolic acid
This stain differentiates bacterial cells based on their ability to retain the primary stain, carbol fuchsin,
despite being washed with an acid-alcohol solution.
How does acid fast stain differentiate bacterial cells?
During the staining process, non-acid-fast bacteria lose the carbol fuchsin stain when treated with acid-
alcohol, while acid-fast bacteria retain it.
Color of Acid-Fast Organisms
After the staining process, acid-fast organisms appear red or pink, while non-acid-fast organisms take on
the color of the counterstain, which is typically blue (like methylene blue).
Name an acid-fast organism
Mycobacterium tuberculosis
Mycobacterium leprae
Is a Schaeffer-Fulton stain a differential stain or a simple stain?
Differentiation stain
Why is Schaeffer Fulton stain a differential stain
it allows for the differentiation between vegetative bacterial cells and endospores based on their
structural characteristics and staining properties.
Malachite green: specifically stains endospores
Safranin: Stains the vegetative cells
What color do endospores turn after Schaeffer Fulton stain
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