Gel Electrophoresis LAB 4 Latest
Update Graded A+
How do we prepare our samples before they hit the gel?
Samples are mixed with loading dye to add color and density, ensuring they sink into the
wells and are visible during the run.
What magical ingredient makes the gel strong enough to h...
Gel Electrophoresis LAB 4 Latest
Update Graded A+
How do we prepare our samples before they hit the gel?
✔✔Samples are mixed with loading dye to add color and density, ensuring they sink into the
wells and are visible during the run.
What magical ingredient makes the gel strong enough to hold DNA?
✔✔Agarose is the magical ingredient that forms the gel, providing a matrix that helps separate
DNA based on size.
How does the DNA fragment size affect its journey through the gel?
✔✔Smaller DNA fragments travel faster and farther through the gel, while larger fragments
struggle to move through the porous matrix.
Why is it essential to set up a control sample in Lab 4?
✔✔A control sample acts as a benchmark, helping to verify that the experiment is running
correctly and that results are reliable.
1
, What might happen if the voltage during electrophoresis is set too high?
✔✔If the voltage is too high, the gel can overheat, causing distortion in the bands or even
damaging the gel structure.
How does the running buffer serve as a friendly environment for DNA?
✔✔The running buffer maintains the pH and provides ions, creating a conductive environment
that allows DNA to migrate smoothly.
What does it mean if your DNA bands appear fuzzy on the gel?
✔✔Fuzzy bands indicate that the DNA may have degraded or that there was too much DNA
loaded, leading to poor separation.
How do we visualize the DNA after it has traveled through the gel?
✔✔DNA is visualized by staining with a dye like ethidium bromide or SYBR Safe, which
fluoresces under UV light, illuminating the bands.
What is the significance of a DNA ladder in your gel?
✔✔The DNA ladder serves as a reference, allowing you to compare the size of your DNA
fragments against known lengths to determine their sizes.
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