(D311 NURS 1010) Microbiology With Lab - Comprehensive FA Guide Q & S 2024(D311 NURS 1010) Microbiology With Lab - Comprehensive FA Guide Q & S 2024(D311 NURS 1010) Microbiology With Lab - Comprehensive FA Guide Q & S 2024
,1. Which of the following techniques is most effective for
isolating a pure culture from a mixed microbial population?
- A) Streak Plate Method
- B) Pour Plate Method
- C) Spread Plate Method
- D) Broth Culture Method
Answer: A) Streak Plate Method
Rationale: The streak plate method is commonly used
to separate individual colonies from a mixed microbial
sample, allowing for the isolation of pure cultures.
2. Which phase of bacterial growth is characterized by the
rapid increase in cell numbers?
- A) Lag Phase
- B) Logarithmic (Exponential) Phase
- C) Stationary Phase
- D) Death Phase
Answer: B) Logarithmic (Exponential) Phase
Rationale: During the logarithmic phase, bacteria
multiply at an exponential rate, leading to a rapid increase
in cell numbers.
, Answer: B) Filtration
Rationale: Filtration is suitable for sterilizing heat-
sensitive solutions as it removes microbes without involving
high temperatures.
### Fill-in-the-Blank Questions
4. The enzyme ________ is pivotal for the polymerase
chain reaction as it withstands high temperatures necessary
for DNA denaturation.
Answer: Taq polymerase
Rationale: Taq polymerase is derived from the
thermophilic bacterium Thermus aquaticus and is heat-
stable, making it ideal for PCR.
5. In Gram staining, _________ serves as the mordant
that fixes the crystal violet stain in Gram-positive bacteria.
Answer: Iodine
Rationale: Iodine combines with crystal violet to form a
complex that is retained in thick peptidoglycan layers of
Gram-positive bacteria.
### True/False Questions
6. True or False: Antibiotic susceptibility testing is
performed using the serial dilution method only.
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