Micro 2420: Lecture
Exam 2 with complete
verified solutions
Describe the life cycle of a lytic bacteriophage such as T4.
Lytic bacteriophages attach through tail fibers. This can be highly specific.
Viral enzymes start to break down the cell wall and the phage injects its
genetic material directly into the cell along with viral enzymes that will
degrade the host DNA. The virus uses the cellular machinery and
degraded host DNA to synthesize viral components. Viral components
assemble into new viruses, although the mechanism is unclear. The virions
are released by lysing the cell, which is accomplished by the lysozyme
continuing to act to weaken the cell wall until the cell bursts.
Why is lysogeny advantageous to a bacteriophage?
The genetic material of the bacteriophage is amplified many times over
that seen in a lytic phage.
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Which of the following bacterial genera produce(s) endospores?
both Bacillus and Clostridium
Methods of microbial control called ________ arrest the growth of microbes.
germistatic
A northern blot differs from a Southern blot in the
type of nucleic acid being isolated.
Which of the following is an example of pasteurization?
Heat is used to kill potential pathogens in apple juice.
The genus Mycobacterium includes species responsible for
tuberculosis
Pleomorphic bacteria
, vary in size and shape.
The enzyme reverse transcriptase
synthesizes DNA from an RNA template.
A(n) ________ is a bacterial virus which is integrated into the host cell DNA.
prophage
Sister chromatids separate and move toward the poles of the cell during
________ of mitosis.
anaphase
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Which of the following characteristics distinguish the archaea from the
bacteria?
cell wall composition, cytoplasmic membrane lipids, and rRNA sequences
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Multiple mitoses followed by a single cytokinetic event is known as
schizogony.
The ________ is the entire interwoven mass of one multicellular fungal
organism.
mycelium
Plaque assays are used for
determining the density of phage in a culture.
In what ways do viruses differ from other pathogens?
Viruses lack cytoplasm and organelles.
Euglenids have an organelle known as a(n) ________ which has a role in
their phototactic behavior.
eyespot
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