ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
Based on the data presented in the table above and with a
prior probability (PP) of 0.5, the probability of paternity
(POP) in this case is:
92.5%
99.5%
90.5%
95.5% - <<,Answers>>>92.5%
This restriction enzyme "digests and removes a methyl group
from Adenine": - <<,Answers>>>Type I
Which method uses 2 pairs of primers to amplify same target
in 2 separate reactions? - <<,Answers>>>nested PCR; highly
specific
useful when specimens are limited in quantity and quality
What method is uses dilutions of known positive standards to
determine relationship between starting target copy/cell
number and cycle number at which fluorescence crosses a
threshold (Ct) - <<,Answers>>>qPCR
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
qPCR usually uses _____ fluorescence and can measure
_____. - <<,Answers>>>SYBR Green; copy number of
genes, viral load, tumor load, effects of treatment
What method exhibits fluorescence when the fluorophore is
removed from a quencher when hairpin structure is overcome
by hybdrization of primer sequence? -
<<,Answers>>>scorpion
Branched DNA is a type of _____ amplification. It is used for
quantitative and qualitative detection of _____. -
<<,Answers>>>signal; hepatitis B, C and HIV-1
Denatured target nucleic acid is bound to capture probes fixed
to a plate well. Extender/preamplifier probes then bind to
captured target via complementary binding. Alkaline
phosphatase-labeled nt bind to each extender probe.
Dioxetane added as a substrate for alkaline phosphatase.
Chemiluminescence is measured. This method is _____. -
<<,Answers>>>bDNA
What will increase yield/lower stringency in PCR? -
<<,Answers>>>decrease: annealing temp, extension temp,
GC content
increase: annealing time, extension time, # cycles, MgCl2
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
What will decrease nonspecific binding/higher stringency in
PCR? - <<,Answers>>>decrease: annealing time, extension
time, # cycles, MgCl2
increase: annealing temp, extension temp
Formamide _____ Tm by disrupting H-bonds. -
<<,Answers>>>lowers
Denaturing HPLC is used for what analysis and what does it
detect? - <<,Answers>>>heteroduplex analysis; detects
polymorphisms/SNPS
What disease is commonly associated with EBV? -
<<,Answers>>>post-transplant lymphoproliferative disease
(PTLD)
Translocations can be detected by _____. -
<<,Answers>>>karyotype, Southern blot, FISH, PCR, RT-
PCR
Use _____ tubes for blood collection for most nucleic acid
assays involving enzymatic treatment of sample. -
<<,Answers>>>EDTA and/or ACD
, ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
If RNA is your target, you must use special tubes containing
_____ for samples. - <<,Answers>>>RNA stabilizing agents
Viral RNA in whole blood should be stored at ____ (temp). -
<<,Answers>>>Room temp
Human RNA in blood/bone marrow should be processed
within _____ (time frame). - <<,Answers>>>24 hours
Purified isolated DNA stable at RT for _____ in fridge or
_____ in freezer. - <<,Answers>>>several months to a year
at least 10 years
Purified RNA is stable at _____ for up to a year or at _____
for several months. - <<,Answers>>>-80C; -20C
M. tuberculosis is resistant to _____. -
<<,Answers>>>isoniazid, rifampin and streptomycin.
CYP2C9*1 metabolizes warfarin _____. -
<<,Answers>>>normally
Answers
Based on the data presented in the table above and with a
prior probability (PP) of 0.5, the probability of paternity
(POP) in this case is:
92.5%
99.5%
90.5%
95.5% - <<,Answers>>>92.5%
This restriction enzyme "digests and removes a methyl group
from Adenine": - <<,Answers>>>Type I
Which method uses 2 pairs of primers to amplify same target
in 2 separate reactions? - <<,Answers>>>nested PCR; highly
specific
useful when specimens are limited in quantity and quality
What method is uses dilutions of known positive standards to
determine relationship between starting target copy/cell
number and cycle number at which fluorescence crosses a
threshold (Ct) - <<,Answers>>>qPCR
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
qPCR usually uses _____ fluorescence and can measure
_____. - <<,Answers>>>SYBR Green; copy number of
genes, viral load, tumor load, effects of treatment
What method exhibits fluorescence when the fluorophore is
removed from a quencher when hairpin structure is overcome
by hybdrization of primer sequence? -
<<,Answers>>>scorpion
Branched DNA is a type of _____ amplification. It is used for
quantitative and qualitative detection of _____. -
<<,Answers>>>signal; hepatitis B, C and HIV-1
Denatured target nucleic acid is bound to capture probes fixed
to a plate well. Extender/preamplifier probes then bind to
captured target via complementary binding. Alkaline
phosphatase-labeled nt bind to each extender probe.
Dioxetane added as a substrate for alkaline phosphatase.
Chemiluminescence is measured. This method is _____. -
<<,Answers>>>bDNA
What will increase yield/lower stringency in PCR? -
<<,Answers>>>decrease: annealing temp, extension temp,
GC content
increase: annealing time, extension time, # cycles, MgCl2
,ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
What will decrease nonspecific binding/higher stringency in
PCR? - <<,Answers>>>decrease: annealing time, extension
time, # cycles, MgCl2
increase: annealing temp, extension temp
Formamide _____ Tm by disrupting H-bonds. -
<<,Answers>>>lowers
Denaturing HPLC is used for what analysis and what does it
detect? - <<,Answers>>>heteroduplex analysis; detects
polymorphisms/SNPS
What disease is commonly associated with EBV? -
<<,Answers>>>post-transplant lymphoproliferative disease
(PTLD)
Translocations can be detected by _____. -
<<,Answers>>>karyotype, Southern blot, FISH, PCR, RT-
PCR
Use _____ tubes for blood collection for most nucleic acid
assays involving enzymatic treatment of sample. -
<<,Answers>>>EDTA and/or ACD
, ASCP MB: Extra-Practice(2024-2025) Questions with
Answers
If RNA is your target, you must use special tubes containing
_____ for samples. - <<,Answers>>>RNA stabilizing agents
Viral RNA in whole blood should be stored at ____ (temp). -
<<,Answers>>>Room temp
Human RNA in blood/bone marrow should be processed
within _____ (time frame). - <<,Answers>>>24 hours
Purified isolated DNA stable at RT for _____ in fridge or
_____ in freezer. - <<,Answers>>>several months to a year
at least 10 years
Purified RNA is stable at _____ for up to a year or at _____
for several months. - <<,Answers>>>-80C; -20C
M. tuberculosis is resistant to _____. -
<<,Answers>>>isoniazid, rifampin and streptomycin.
CYP2C9*1 metabolizes warfarin _____. -
<<,Answers>>>normally
