Exam (elaborations)
MB(ASCP) EXAM () PRACTICE
- Course
- Institution
MB(ASCP) EXAM () PRACTICE
[Show more]
Seller
Follow
Content preview
MB(ASCP) EXAM (2024-2025) PRACTICEmethods of gel staining: - <<<Answers>>>1. EtBr
2. silver staining
3. SYBR green
4. Methylene Blue
SYBR Green I and II - <<<Answers>>>I= ds DNA
II= ss and RNA
3 steps of translation - <<<Answers>>>1. initiation
2. elongation
3. termination
mode of inheritance for Huntington's disease -
<<<Answers>>>autosomal dominant
Huntington's disease - <<<Answers>>>chr 4p16
IT-15 Gene (Huntington protein)
CAG repeats
<29 = normal
29-35 = borderline
36-121 = disease
, MB(ASCP) EXAM (2024-2025) PRACTICE
mode of inheritance for cystic fibrosis -
<<<Answers>>>autosomal recessive
gene impacted in CF - <<<Answers>>>CFTR gene
Fragile X - <<<Answers>>>most common form of inherited
retardation
-FMR-1 gene
-Xq27.3
-CGG repeat
5-44 = normal
59-200 = carrier
200+ = disease
nitrocellulose membranes - <<<Answers>>>fragile, brittle
NOT for re-probing
intense color
Nylon membrane - <<<Answers>>>strong, durable
can strip and re-probe
can be charged
NOT for Western Blotting
, MB(ASCP) EXAM (2024-2025) PRACTICE
Southern Blot - <<<Answers>>>DNA
Western Blot - <<<Answers>>>protein
Northern Blot - <<<Answers>>>RNA
steps of pre-transfer for membranes - <<<Answers>>>1. de-
purinate DNA to reduce the size of fragments to transfer
2. denature DNA
3. neutralize to equlibrate gel and membrane
capillary transfer to membrane - <<<Answers>>>transfer
buffer drawn up through a reservoir and passes through gel
into a stack of paper towels weighted; DNA moves from gel
with movement of buffer
does NOT work forpolyacrylamide gels
dot blots - <<<Answers>>>bind DNA or RNA to membrane
without first separating the components by electrophoresis
less discrimination in hybridization
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller jackbundi9071. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77254 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now