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bacteriology exam 1 questions with complete solutions

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bacteriology exam 1 questions with complete solutions

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  • October 29, 2024
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  • 2024/2025
  • Exam (elaborations)
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  • Bacteriology
  • Bacteriology
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BravelRadon
bacteriology exam 1 questions with
complete solutions

how is the genome different in pro- v eu-karyotes - correct answer ✔✔prokaryotes: 1 chromo, may have
extrachromosomal DNA =plasmids

eukaryotes: mult chromosomes, extrachromasomal DNA = mitochondria



what is the structure of organelles in pro- v eu-karyotes - correct answer ✔✔pro: DO NOT have
membrane bound organelles in the cytoplasm

eu: do have membrane bound organelles --> nucleus, ER, golgi app



what are the basic external structures of a bacteria - correct answer ✔✔surface layers: S layer + capsule

appendages: flagella, pili, fimbriae



what are the boundaries of a bacteria - correct answer ✔✔-outer membrane

-cell wall

-cytoplasmic membrane



what is the fx of the cell wall - correct answer ✔✔maintains integretity of the cell (eg keeping osmotic
pressure in check)



*the only one that doesnt have this is mjicroplasma



what is the fx of the outer membrane - correct answer ✔✔-facilitates nutrient acquisition

-virulence

-protection



**some have, not all

,what is the fx of the cytoplasmic membrane - correct answer ✔✔(fragile, needs protection)

-controls nutrients coming in and out of the sytoplasm

-needs protection! this is why the cell wall is impt



what are the internal components of a bacteria - correct answer ✔✔-cytoplasm

-ribosomes

-inclusion

-nucleoid/chromosome

-cytoskeleton

-endospore

-plasmid



what is the main difference between gram pos and gram neg bacteria - correct answer ✔✔the
components of the cell wall (one can be decolorized and restained (neg), the other cant (pos)



what is the comp of the cell wall for a gram pos bacteria - correct answer ✔✔-they have a cell
membrane

-they have a large/thick peptidoglycan layer

-the surface proteins are on top of the peptidoglycan layer



-gives rigitiy and cell shape

-protects from osmotic challenge and some toxic compounds



what is the comp of the cell wall of a gram neg bacteria - correct answer ✔✔-cell membrane

-thin peptidoglycan layer

-lipoprotein layer

-outermembrane = lipopolysaccharide (LPS) with surface proteins in it)

-can have porins --> allow molecules into the cell (hydrophilic) and eject molecules out

,*uses the other membrane (LPS) for protection



what are the 2 main shapes of bacteria? - correct answer ✔✔rods and cocci (spherical)

-generally 1-10um in length and 0.2-1 um in width

-but they can come in many shapes and sizes



how do we commonly group bacteria? - correct answer ✔✔using gram status and shape

-gram pos rods

-gram pos cocci

-gram neg rods



*dont really see gram neg cocci in vet med but are mucosal flora



what are different examples of bacterial attachement - correct answer ✔✔-fimbriae

-biofilm

-capsule



what is fimbriae - correct answer ✔✔-protein structures on the outside of the cell

-fine, harilike that faciliate adhesion through "grappling hook-like" mechanism

-usually distributed all over the cell surface

-can aid in ID



*amino acid comp of the proteins may be changed in oder to evade immune syst (good target Ag for
immune cells)



what is biofilm - correct answer ✔✔-aggregate of bacteria and extracellular components

-forming complex comminites, often a single spp but not always

, -helps bacteria survive in adverse conditions (decreases immune response, anti microbial drugs are
unable to penetrate through matrix well)



*occurs on implants and invasive devices, in tussues



what is a capsule - correct answer ✔✔in addition to a cell wall, they can have a layer external to cell wall
with mult functions. usually comp of polysaccharides and tightly bound to the cell wall)

-avoid phagocytosis

-resistance to toxic substances

-adherence



what is the S layer - correct answer ✔✔the "slime layer"

-less robust than the capsule

-monolayer of protein used for protection and attachment

-loose structure and loosely adhered to cell (can wash away, but still helpful for protection and
attachment)



what are types of tools do bacteria use to move - correct answer ✔✔-flagella



what is flagella - correct answer ✔✔-long round filaments embedded into cell wall (change direction
with change in rotation

-facilitates spread in response to chemotactic signals (with in environment)

-can be used to ID bact



what are bacterial mechanisms of DNA exchange - correct answer ✔✔-conjugation

-tranformation

-transduction



what is pilus - correct answer ✔✔-mechanism for bacterial cell to cell sharing of chromosomal or
plasmid DNA called conjugation

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