Exam (elaborations)
Clinical Bacteriology; Exam 1 review fully solved
- Course
- Institution
Clinical Bacteriology; Exam 1 review fully solved
[Show more]
Content preview
Clinical Bacteriology; Exam 1 reviewfully solved
What are the components of the gram stain? - correct answer ✔✔Peptidoglycan
Found in all bacteria except mycoplasma
Gram positive have a higher concentration that Gram negative
Hydrolyzed by lysosome which causes lysis
Mycolic acids
Composed of long branched beta-hydroxyl acids; water insoluble
Resistant to penetration; acid fast staining properties in sufficient quantities
Found in mycobacteria and Nocardia
Techoic acid
Only in gram positive bacteria
Major surface antigens when present
LPS
Endotoxin; only in gram negative bacteria; released upon death
Lipid + polysaccharide
Gram staining - correct answer ✔✔Differentiates between Gram positive bacteria (blue or purple) and
Gram negative bacteria (red or pink)
Colonies of cells are mixed with a crystal violet dye (primary stain), allowed to sit with a binding agent
(Grams Iodine), washed with a decolorizer (alcohol), and stained with a secondary stain (safranin)
Gram positive bacteria have a thicker band of peptidoglycan which has a high affinity for crystal violet so
it holds onto the dye and is not easily washed out; it retains the blue color of the dye, and remains blue
or purple
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller BravelRadon. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75632 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now