Tentamen (uitwerkingen)
BIO 219 Final Exam: Questions With Accurate Solutions
- Vak
- Instelling
BIO 219 Final Exam: Questions With Accurate Solutions
[Meer zien]Voorbeeld 3 van de 22 pagina's
Voorbeeld 3 van de 22 pagina's
In winkelwagen
Voorbeeld van de inhoud
BIO 219 Final Exam: Questions With AccurateSolutions
What is Aliivibrio fischeri (A. fischeri)? Right Ans - Rod shaped bacteria;
gram negative
Where does Aliivibrio fischeri (A. fischeri) live? Right Ans - In symbiosis
with many marine organisms.
What is special about Aliivibrio fischeri's (A. fischeri) chromosomal DNA?
Right Ans - Has the lux operon -> codes for proteins that are needed for
bioluminescence.
What is E. coli? Right Ans - Rod shaped bacteria; gram negative
Where does E. coli live? Right Ans - lower gut of warm-blooded animals
What is E. coli used for? Right Ans - Prokaryotic model organism for
biotechnology and microbiology as the host for working with recombinant
DNA.
Gram Negative vs. Gram Positive: Right Ans - External Lipopolysaccharide
layer w/ a single layer of peptidoglycan
How does gram staining work? Right Ans - Peptiodglycan layer -> gram
staining
Thin layer in neg. cells results in no staining
LPS can vary based on composition and length of O-antigen as well as
composition of core polysaccharides
What is an operon? Right Ans - genetic regulatory system; genes that are
coding for functionally related proteins are clustered w/ DNA.
This means proteins synthesis is controlled coordinately in response to
cellular needs.
,What is chromosomal DNA? Right Ans - Large circular DNA in bacteria.
What is restriction digestion? Right Ans - enzymatic reactions -> cut DNA
into smaller pieces.
has enzymes that cut the DNA at only specific sequences.
What are plasmids? Right Ans - small circular, usually double-stranded
DNA that is physically separate from chDNA.
carry "survival genes" -> antibiotic resistance
T/F: Plasmids are able to replicate autonomously within the cell and can be
transferred from one organism to another. Right Ans - True
How can plasmids be transferred from one organism to another? Right Ans
- Transformation, transduction, and conjugation.
What is ligation? Right Ans - enzymatic reaction -> pastes 2 pieces of DNA
together (from same or diff organisms).
What is recombinant DNA? Right Ans - When DNA from two sources comes
together -> chDNA + Plasmid DNA.
What is shotgun cloning? Right Ans - randomly digesting a large piece of
DNA to smaller pieces
Why is shotgun cloning done? Right Ans - so it can be ligated into plasmids
for transport to other organisms.
What is a genomic library? Right Ans - collection of cloned DNA fragments
from a genome where each part of the genome is represented in the library.
can use to screen for sequence of interest
What is a clone? Right Ans - A bacteria housing a recombinant plasmid that
contains a piece of genomic DNA
may or may not have gene of interest.
, Why is bioluminescence beneficial to fish? Right Ans - Communication
Attracting Prey
Hiding from predators
Attracting a Mate
Why is bioluminescence beneficial to bacteria? Right Ans - Reliable food
source
-Glucose
-Amino Acids
How is bioluminescence completed via cooperative development? Right
Ans - Infection by bacteria at birth → light organ maturation
Ex. Lophius piscatorius (angler fish)
What are some examples of species that host A. fischeri? Right Ans -
Monocentris japonicus (pinecone fish)
Euprymna scolopes (Hawaiian bobtail squid)
What are luciferins? Right Ans - are light emitting hetero-cyclic compounds
that undergo enzyme catalyzed oxidation
leads to emission of light upon decaying to a ground state
origination -> mechanism to deal with oxidative stress.
What are some dangerous photochemically generated oxygen species marine
animals are exposed to? Right Ans - superoxide anion (O2-) and hydrogen
peroxide (H2O2) -> Causes damage to DNA and initiates cellular lysis
With increasing ocean depth, there is a decrease in concentrations. How is the
pressure maintained? Right Ans - Symbiotic relationships maintain the
pressure to conserve bioluminescence.
What causes bioluminescence? Right Ans - Oxidation of luciferin catalyzed
by luciferase
Blue-green light emitted at 490 nm
Voordelen van het kopen van samenvattingen bij Stuvia op een rij:
Verzekerd van kwaliteit door reviews
Stuvia-klanten hebben meer dan 700.000 samenvattingen beoordeeld. Zo weet je zeker dat je de beste documenten koopt!
Snel en makkelijk kopen
Je betaalt supersnel en eenmalig met iDeal, creditcard of Stuvia-tegoed voor de samenvatting. Zonder lidmaatschap.
Focus op de essentie
Samenvattingen worden geschreven voor en door anderen. Daarom zijn de samenvattingen altijd betrouwbaar en actueel. Zo kom je snel tot de kern!
Veelgestelde vragen
Wat krijg ik als ik dit document koop?
Je krijgt een PDF, die direct beschikbaar is na je aankoop. Het gekochte document is altijd, overal en oneindig toegankelijk via je profiel.
Tevredenheidsgarantie: hoe werkt dat?
Onze tevredenheidsgarantie zorgt ervoor dat je altijd een studiedocument vindt dat goed bij je past. Je vult een formulier in en onze klantenservice regelt de rest.
Van wie koop ik deze samenvatting?
Stuvia is een marktplaats, je koop dit document dus niet van ons, maar van verkoper Studyhall. Stuvia faciliteert de betaling aan de verkoper.
Zit ik meteen vast aan een abonnement?
Nee, je koopt alleen deze samenvatting voor $18.99. Je zit daarna nergens aan vast.
Is Stuvia te vertrouwen?
4,6 sterren op Google & Trustpilot (+1000 reviews)
Afgelopen 30 dagen zijn er 75323 samenvattingen verkocht
Opgericht in 2010, al 14 jaar dé plek om samenvattingen te kopen