Microbiology Lab Final - OSU 4000.01 -
Prelab & Lab Quiz Questions
When you finish using the compound brightfield microscope, what should you have your
lab instructor do?
-Inspect and color your drawings of bacteria
-Inspect your microscope to see if it is properly cleaned
-Clean the microscope
-Collect any slides you prepared during the lab - answer Inspect your microscope to see
if it is properly cleaned
You are allowed to email your lab instructors and TAs any questions about lab related
topics. - answer True
What do you have to do when you finish using the compound brightfield microscope?
-Tell your lab partner to place it at the lab bench for shared materials
-Clean the microscope and ask your lab instructor to inspect it
-Leave it on the bench for the lab coordinator to clean it - answerClean the microscope
and ask your lab instructor to inspect it
Where should you dispose of paper towels used to clean your bench at the beginning
and end of the lab?
-In the trash can located inside the nearest restroom
-In the regular trash can in the lab
-Leave it on your lab bench and the lab coordinator will take care of it
-In the red biohazard bucket - answerIn the regular trash can in the lab
You are allowed to eat and drink water in lab. - answerFalse
Which of the following slides will you view in lab period 1?
,-prepared slide of yeast
-prepared slide of protezoa
-Wet mount of living unstained microbes
-None are correct
-All are correct
-prepared slide of bacteria - answerAll are correct
Which of the objective lense(s) can be used with immersion oil?
-40X
-1000X
-100X
-10X - answer100X
You are sick and cannot go to lab, what do you need to do?
Hint: There may be more than one answer.
-Stay home and do not think about it
-Give a doctor's note to your lab instructor when you are well and attending lab again
-Drink lots of water and ask the lab instructor to delay the lab period for everyone until
you feel better
-Contact the doctor and get a doctor's note to minimize the risk of losing course points
for missing a lab - answer-Contact the doctor and get a doctor's note to minimize the
risk of losing course points for missing a lab
-Give a doctor's note to your lab instructor when you are well and attending lab again
You are required to tie your shoulder-length (or longer) hair back. - answerTrue
You are required to wear a long sleeve lab coat when attending the microbiology labs. -
answerTrue
What is the highest total magnification possible for the compound brightfied
microscope? - answer1000X
,Emily, a student in a microbiology course, got ready to leave home for the third lecture
and lab of the semester. She grabbed her backpack with all the needed material, her
elastic band for to tie her hair back and her flip flops, and headed to school. What did
the student forget?
-Her closed toed shoes
-Her inoculating loop
-Her lab coat
-Nothing: she did everything correctly - answerHer closed toed shoes
The lab instructor entered the lab, took a sip of her water bottle, and pulled up the
PowerPoint presentation on the screen. She then quickly moved the water bottle so it
faced the back of the computer instead of the lab, and then started the lab presentation.
What should she have done differently?
-Inform students that are chewing gum that they have to trash the gum after the lab
presentation, since the lab work will begin shortly.
-Make sure that all students are present before starting the lab presentation.
-Wait to drink from the water bottle until after the presentation.
-Drink from the water bottle before entering the lab and leave the water bottle outside
the lab, before entering.
-Make sure that other students' water bottles are collected and facing the back of the lab
computer.
-Nothing: all she did to prepare for lab was correct. - answerDrink from the water bottle
before entering the lab and leave the water bottle outside the lab, before entering.
Where should you dispose of used swabs in lab?
-all are correct
-red biohazard bucket
-none are correct
-regular trash cans - answerred biohazard bucket
, Which parts of the microscope are used to adjust the amount of light reaching the
specimen?
-Iris diaphragm
-The eyepieces
-The mechanical stage
-none are correct
-The base
-The nose piece - answerIris diaphragm
Which part of the microscope should not be necessary to use once the specimen is in
focus using the 10X objective?
-The base
-The fine focus adjustment knob
-Both the course and fine focus adjustment knobs
-The arm
-The coarse focus adjustment knob
-Iris diaphragm
-The nose piece - answerThe coarse focus adjustment knob
When looking at a specimen using the low power objective lens the total magnification
is what?
-100X
-400X
-1000X
-10X
-40X - answer100X
Which types of microscope and methods of microscopy employ electron beams and
electromagnets to create the magnified image of a specimen?
Prelab & Lab Quiz Questions
When you finish using the compound brightfield microscope, what should you have your
lab instructor do?
-Inspect and color your drawings of bacteria
-Inspect your microscope to see if it is properly cleaned
-Clean the microscope
-Collect any slides you prepared during the lab - answer Inspect your microscope to see
if it is properly cleaned
You are allowed to email your lab instructors and TAs any questions about lab related
topics. - answer True
What do you have to do when you finish using the compound brightfield microscope?
-Tell your lab partner to place it at the lab bench for shared materials
-Clean the microscope and ask your lab instructor to inspect it
-Leave it on the bench for the lab coordinator to clean it - answerClean the microscope
and ask your lab instructor to inspect it
Where should you dispose of paper towels used to clean your bench at the beginning
and end of the lab?
-In the trash can located inside the nearest restroom
-In the regular trash can in the lab
-Leave it on your lab bench and the lab coordinator will take care of it
-In the red biohazard bucket - answerIn the regular trash can in the lab
You are allowed to eat and drink water in lab. - answerFalse
Which of the following slides will you view in lab period 1?
,-prepared slide of yeast
-prepared slide of protezoa
-Wet mount of living unstained microbes
-None are correct
-All are correct
-prepared slide of bacteria - answerAll are correct
Which of the objective lense(s) can be used with immersion oil?
-40X
-1000X
-100X
-10X - answer100X
You are sick and cannot go to lab, what do you need to do?
Hint: There may be more than one answer.
-Stay home and do not think about it
-Give a doctor's note to your lab instructor when you are well and attending lab again
-Drink lots of water and ask the lab instructor to delay the lab period for everyone until
you feel better
-Contact the doctor and get a doctor's note to minimize the risk of losing course points
for missing a lab - answer-Contact the doctor and get a doctor's note to minimize the
risk of losing course points for missing a lab
-Give a doctor's note to your lab instructor when you are well and attending lab again
You are required to tie your shoulder-length (or longer) hair back. - answerTrue
You are required to wear a long sleeve lab coat when attending the microbiology labs. -
answerTrue
What is the highest total magnification possible for the compound brightfied
microscope? - answer1000X
,Emily, a student in a microbiology course, got ready to leave home for the third lecture
and lab of the semester. She grabbed her backpack with all the needed material, her
elastic band for to tie her hair back and her flip flops, and headed to school. What did
the student forget?
-Her closed toed shoes
-Her inoculating loop
-Her lab coat
-Nothing: she did everything correctly - answerHer closed toed shoes
The lab instructor entered the lab, took a sip of her water bottle, and pulled up the
PowerPoint presentation on the screen. She then quickly moved the water bottle so it
faced the back of the computer instead of the lab, and then started the lab presentation.
What should she have done differently?
-Inform students that are chewing gum that they have to trash the gum after the lab
presentation, since the lab work will begin shortly.
-Make sure that all students are present before starting the lab presentation.
-Wait to drink from the water bottle until after the presentation.
-Drink from the water bottle before entering the lab and leave the water bottle outside
the lab, before entering.
-Make sure that other students' water bottles are collected and facing the back of the lab
computer.
-Nothing: all she did to prepare for lab was correct. - answerDrink from the water bottle
before entering the lab and leave the water bottle outside the lab, before entering.
Where should you dispose of used swabs in lab?
-all are correct
-red biohazard bucket
-none are correct
-regular trash cans - answerred biohazard bucket
, Which parts of the microscope are used to adjust the amount of light reaching the
specimen?
-Iris diaphragm
-The eyepieces
-The mechanical stage
-none are correct
-The base
-The nose piece - answerIris diaphragm
Which part of the microscope should not be necessary to use once the specimen is in
focus using the 10X objective?
-The base
-The fine focus adjustment knob
-Both the course and fine focus adjustment knobs
-The arm
-The coarse focus adjustment knob
-Iris diaphragm
-The nose piece - answerThe coarse focus adjustment knob
When looking at a specimen using the low power objective lens the total magnification
is what?
-100X
-400X
-1000X
-10X
-40X - answer100X
Which types of microscope and methods of microscopy employ electron beams and
electromagnets to create the magnified image of a specimen?
