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Bioc 2580 Midterm Exam Study Guide Solutions very non-polar side chains - ANSWER-ala, val, leu, ile, met, phe - side chains are dominated by non-polar and hydrophobic hydrocarbons moderately non-polar - ANSWER-gly, cys, pro, tyr, trp -gly: single H, not enough to be very non-polar -cys: sligh...

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  • November 3, 2024
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Bioc 2580 Midterm Exam Study Guide

Solutions


very non-polar side chains - ANSWER✔✔-ala, val, leu, ile, met, phe


- side chains are dominated by non-polar and hydrophobic hydrocarbons


moderately non-polar - ANSWER✔✔-gly, cys, pro, tyr, trp


-gly: single H, not enough to be very non-polar


-cys: slightly polar SH


-pro: side chain links to N and alpha C, polar non moderates non-polar hydrocarbon


-tyr & trp: single polar group that partly offsets very non-polar benzene


polar uncharged - ANSWER✔✔-ser, thr, asn, gln


-ser & thr: side chains with OH


asn & gln: polar amide groups


-don't hain or lose H+ in aqueous solution at pH 7, so they're uncharged


-good H bond acceptors


very polar positively charged - ANSWER✔✔-His, lys and arg


- contain weak bases that gain H+


-charge makes them very polar, overriding non-polar hydrocarbons

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very polar negatively charged - ANSWER✔✔--have carboxylic acid groups that lose H+


henderson-hasselbalch equation - ANSWER✔✔-pH=pKa+log [deprotonated] / [protonated]


deprotonated - ANSWER✔✔-pH is one unit higher than pKa


protonated - ANSWER✔✔-pH is one unit below pKa


partition chromatography - ANSWER✔✔--stationary phase that hydrogen bonds to polar AAs


-mobile phase that flows past the particles and is non-polar


-polar AA spend more time hydrogen bonded and move slowly


-nonpolar AA spend more time in solvent and move almost as fast as solvent


thin layer chromatography - ANSWER✔✔--silica gel is spread in a thin layer on a plastic sheet, this is the

polar stationary phase


-samples are applied to lower edge and placed in solvent


-solvent soaks up sheet and non-polar AA move with the solvent


-highest point reached by solvent is the solvent front


-relative mobility: RF=XB (distance travelled by sample) / XF(distance travelled by solvent


-nonpolar AA have high RF (mobile)


-polar AA have low RF (stationary)


column chromatography - ANSWER✔✔--sample is added


-buffer is added, carrying sample out



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