Unit 6 - Organisms respond to changes in their internal and external environments
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Biology CGP’s revision car
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Exam Board: AQA
Revision Question Cards
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9 781789 085921
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, A-Level
Biology
Exam Board: AQA
Revision
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Topics match CGP’s books and
the course specification.
49. RNA and Protein Synthesis (1) Topic 4A — DNA, RNA
Questions on all the
and Protein Synthesis
Quick Questions
key topics, as well as on 1) What does the ‘m’ in mRNA stand for?
2) What is an mRNA codon?
practicals and maths skills. 3) What is transcription?
Now try these:
4) Look at the work of art diagram on the right. A B C
Which structure (A, B or C) represents tRNA?
5) A DNA sequence reads: ATTACG.
What is the complementary mRNA sequence?
6) Describe the role of RNA polymerase in protein synthesis.
7) Explain why eukaryotic mRNA does not contain introns.
8) Chloramphenicol is an antibiotic (a drug that kills or inhibits the growth of bacteria).
It works by binding to ribosomes and inhibiting peptide bond formation.
Explain the effect this will have on protein synthesis.
Topic 4A — DNA, RNA
and Protein Synthesis 49. RNA and Protein Synthesis (1)
ANSWERS
1) The ‘m’ in mRNA stands for messenger. (The ‘RNA’ stands for ribonucleic acid, but you already knew that.)
2) An mRNA codon is a sequence of three mRNA bases that codes for an amino acid. Complete answers
to each question.
3) Transcription is the first stage of protein synthesis, in which an mRNA copy of a gene is made from DNA.
4) Structure B represents tRNA, which folds itself into a lovely clover shape, held together by hydrogen bonds
between specific, complementary bases. Structure A represents mRNA, which is single-stranded. Structure
C represents DNA, which is double-stranded.
5) The complementary mRNA sequence is UAAUGC. In complementary base pairing, specific bases always
pair up — A with T or U and C with G. RNA contains U (uracil) instead of T.
6) RNA polymerase takes part in transcription. It lines up free RNA nucleotides alongside the exposed bases
on a DNA template strand. The enzyme then joins adjacent nucleotides together, via phosphodiester bonds,
to form an mRNA molecule.
7) In eukaryotes, pre-mRNA (containing both introns and exons) is spliced before it leaves the nucleus.
This removes the introns (non-coding regions) and joins together the exons.
8) Chloramphenicol prevents the formation of peptide bonds between amino acids during translation,
so a polypeptide chain (protein) will be unable to form.
TIP
Interesting-ish fact: bacterial ribosomes and eukaryotic ribosomes have slightly different structures. This means
Handy tips on every card.
chloramphenicol can be used to inhibit bacterial protein synthesis without affecting human protein synthesis.
Quick Questions
1) What is a monomer? Give three examples of monomers.
2) What is a disaccharide?
3) Which chemical elements are found in all carbohydrates?
Now try these: CH2OH
H C O OH
4) There are two different isomers of glucose. H
Name the isomer of glucose shown on the right and describe C HO H C
how its structure differs to the other isomer of glucose. HO H
C C
5) What type of reaction breaks down disaccharides? H OH
Explain how this reaction works and give an example of this reaction.
6) The Benedict’s test can be used to test for the presence of sugars. Describe how you would
test for a reducing sugar and what results you would expect to see in a positive test.
7) Describe how you would test for the presence of non-reducing sugars.
Mono- means “one”, di- means "two" and poly- means "many". This makes it easier to remember things like two
TIP
monosaccharides join up to make a disaccharide and that many monosaccharides make up a polysaccharide.
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