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BIO 340 Module 5 Questions And Verified Answers A+ Graded
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BIO 340 Module 5 Questions And VerifiedAnswers A+ Graded
What is the correct order of the enzyme action in DNA replication? Label steps 1 to 7. -
ANSWER 1. Bind to origin of replication (AT rich sequence)
2. Helicase/topoisomerase action
3. Single-stranded binding proteins bind and
block reannealing
4. Priming, or synthesis of RNA primers
5. DNA polymerization/proofreading
6. 5'-3' exonuclease activity/DNA
polymerization
7. Ligation
Transcribe and Translate the following DNA strand
DNA 3' T A C A T A A C C A T T 5' - ANSWER 5' AUGUAUUGGUAA
The haploid human genome is 3 Gbp long (3x10^9 bp). How long would the genomic
DNA in a single diploid cell in your body be if it were stretched out end to end? -
ANSWER -2m
The haploid human genome is 3 Gbp in length (3x109 bp). There are ~5x1013 cells in the
human body. How long would all of the human genomic DNA in your body be if it were
stretch out end to end? - ANSWER 100 billion Km
Over what temperature range does a DNA allow the binding of primers? - ANSWER
45-68
, What are the three major parts of a DNA nucleotide? - ANSWER A phosphate group, a
nitrogenous base, a deoxyribose
Hydrogen bonds are weaker than covalent bonds. Hydrogen bonds are broken in the
process of PCR during the denaturation step. - ANSWER true
T or F about DNA Adenine pairs with Uracil. - ANSWER False
T or F Two strands of DNA Double helix are held together by a weak bond. - ANSWER
True
You are running a PCR and want to estimate about how much DNA you could have after
30 cycles on the thermocycler. - ANSWER 2.14 x 109
What are all the necessary reagents required for the PCR - ANSWER 1. Double stranded
DNA complex with target sequence
2. Deoxyribose nucleoside triphosphate
3. Heat stable DNA polymerase
4. two nonidentical single strand DNA priming sequences (forward and reverse)
5. Buffer
Denaturation - ANSWER heat the mixture to 95° C this will separate the double stranded
DNA into two single strands
Primer Annealing (PCR) - ANSWER the reaction temperature is lowered to 48-68° C. This
allows primers to create bonds with their complementary sequences of the target DNA
Extension/ Elongation - ANSWER the temperature is raised to 72° C. This allows
polymerase to synthesize DNA accurately and rapidly
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