Genetic Technology Exam Questions
and Answers
What is Gel Electrophoresis? - Answer-Method of separating DNA, RNA, and protein
fragments based on size
Which fragments move the furthest during gel electrophoresis? - Answer-Smaller
Fragments
The DNA fragments move towards which end of a gel during gel electrophoresis? -
Answer-The positively charged end because DNA is negatively charged.
What is Cloning? - Answer-Creation of an organism that is genetically identical to
another organism
How do you clone an organism? - Answer-You take the nucleus of a somatic cell and
fuse it with an egg without a nucleus, then stimulate replication to create the organism
What is PCR? - Answer-PCR stands for polymerase chain reaction. It can take a small
portion of DNA and make thousands of copies of it.
How is PCR used? - Answer-PCR is used every day to diagnose diseases, identify
bacteria and viruses, match criminals to crime scenes, and in many other ways
What is genetic engineering? - Answer-Unnatural modification of an organisms genetic
makeup.
What are transgenic organisms? - Answer-Organisms who have had their DNA altered,
foreign DNA inserted. Also called GMO's (Genetically Modified Organisms)
What are some examples of transgenic organisms? - Answer-Plants who have been
modified to produce their own insecticide or resist herbicides.
Plants can also be altered to increase their vitamin or protein values.
Animals such as goats can also be modified to change nutrients in their milk.
What is Recombinant DNA? - Answer-DNA that has been combined with foreign DNA.
What is an example of Recombinant DNA? - Answer-The gene for production of Human
Insulin has been inserted into bacteria in order to force these bacteria to create Human
Insulin.
Selective Breeding - Answer-Animals are chosen for desired traits to produce offsprings
with those particular traits
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