Class notes
Spectroscopy lab report
- Course
- CHM 1045L (CHM1045L)
- Institution
- Florida International University
Lab report on the spectroscopy lab with calculations, tables, and graphs
[Show more]
Seller
Follow
Content preview
Name of the lab report: SpectroscopyStudent’s Name:
Panther ID:
CHM1045L
Section number:
TA name:
Introduction:
This experiment delves into spectophotometry principles, a fundamental analytical chemistry
technique. According to Eyring (2014), spectrophotometry is a technique used to measure the
intensity of light absorbed or transmitted by a substance as a function of wavelength. This
light absorption is governed by Beer’s Law, which states that a solution's absorbance (A) is
directly proportional to the concentration of the absorbing species and the path length of the
sample. According to Edinburgh Instruments (2023), Beer’s Law is expressed as A= εcl,
where A is the absorbance, ε is the molar absorptivity, c is the concentration of the absorbing
species and l is the path length of the sample.
The key terms involved in spectrophotometry include absorption and absorbance. According
to Molecular Devices (2023), absorption refers to the process by which light energy is
absorbed by a substance, causing electrons to transition to higher energy levels. Absorbance,
on the other hand, is a measure of the amount of light absorbed by a solution and is directly
related to the concentration of the absorbing species.
, To obtain the maximum absorbance of the analyte, the concept of analytical wavelength is
used, which is a specific wavelength chosen at which the absorbance of the analyte is
measured to ensure optimal sensitivity and accuracy in the analysis.
To quantify the relation between absorbance and concentration, a calibration curve is
constructed. This curve is generated by measuring the absorbance of standard solutions with
known concentrations across a range of wavelengths. A linear relationship can be established
by plotting absorbance versus concentration, allowing for the determination of unknown
concentrations based on their absorbance values.
This experiment uses spectrophotometry and Beer’s Law to quantitatively determine the
concentration of an analyte in solution by measuring its absorbance at a specific wavelength.
By understanding these fundamental principles, effective analysis and interpretation can help
achieve analytical goals.
The below table provides information on the data used in the procedure and calculation of the
concentration for unknown solutions.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller ssrp05. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75057 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now