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Exam of 2 pages for the course MACS at MACS (MACS quiz)
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MACS quizWhich of the following are true about MACS beads? - answer all of the above
Separation using MACS beads requires - answer magnetic field
A disadvantage of using direct labeling in MACS separation is - answer activation of
cells
Direct labeling yields high purity cell populations. - answer True
Examples of indirect labeling involve - answer all of the above
Indirect labeling is useful in cases where the cell surface marker of interest is expressed
in low levels on the target cells. - answer True
Under which will a depletion approach cell separation be used? - answerall of the above
What size of nylon mesh would be appropriate to filter cells to capture debri and
clamped-up cells? - answer30 micrometers
An ice bucket is used during 'staining' protocols that keeps cells at ~4 degrees celcius.
This temperature - answerdecreases endocytosis of cell surface markers
'Degasing' buffers is recommended in MACS staining because it reduces bubble
formation and allows for better staining. - answerTrue
Cloning cells in tissue culture - answerall of the above
A cell culture that contains a 10% cell contaminant that divides every 24 hours caneasily
surpass the number of cells of interest - answerin 10 days
Cloning of primary cells is difficult due to - answersenescence (Deterioration associated
with age in cells)
Cloning of attached/adherent cells can be carried out in - answerall of the above
Cloning of cells in suspension can be carried out using a viscous matrix such as
methocel. - answertrue
Dilution cloning requires dilution of a cell supspension and subsequent culturing that will
result in - answerdistinct colonies
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