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BI 234 LAB PRACTICAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BY EXPERT $10.49   Add to cart

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BI 234 LAB PRACTICAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BY EXPERT

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BI 234 LAB PRACTICAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BY EXPERT Gram negative bacteria Thin layer of peptidoglycan. Contains outer membrane (lipo-polysaccharides, lipoproteins, phospholipids) with porin. gram positive bacteria Bacteria that have a thick peptido glycan...

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  • November 9, 2024
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  • 2024/2025
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BI 234 LAB PRACTICAL EXAM QUESTIONS AND

ANSWERS WITH COMPLETE SOLUTIONS VERIFIED BY

EXPERT

Gram negative bacteria

Thin layer of peptidoglycan. Contains outer membrane (lipo-polysaccharides,

lipoproteins, phospholipids) with porin.

gram positive bacteria

Bacteria that have a thick peptido glycan cell wall, and no outer membrane. They stain

very darkly (purple) in Gram stain.

endospore stain

A differential stain used to detect the presence and location of spores in bacterial cells.

TSA plate

Tryptic Soy Agar

- universal growth medium

plate streaked for isolation

4 quadrants

Phenylethyl Alcohol Agar (PEA)

Selects for the growth of gram positive microorganisms, because Phenylethyl Alcohol is

inhibitory to the growth of gram negative organisms

, MacConkey Plate

selective medium-growth of gram -, gram + is inhibited

differential medium-fermentation of lactose causes growth

mannitol salt plate

selective medium-growth of halotolerant bacteria

differential medium-mannitol fermenters turns plate yellow

fluid thioglycollate

Slightly viscous broth used for determining the oxygen requirement of different microbes

Catalase Test

used to identify organisms that produce the enzyme catalase

indicator used in thioglycollate broth

Resazurin

color difference allows for confirmation of oxygen and no oxygen areas in the tube

biosafety level

Level (BSL 1-4) or Biosafety containment level describes the specific equipment,

practices and facilities that must be used for containment of biological agents

bsl-1

no special precautions; basic teaching labs

bsl-2

lab coat, gloves, eye protection

example of bsl2 bacteria

salmonela enterica, staphylococcus aureus and HIV

bsl-3

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