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Biol 1020 Lab Final Exam With Complete Solutions Latest Update
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Biol 1020 Lab Final Exam With CompleteSolutions Latest Update
Liquid medium used to enrich the number of microbes. ANSWER Broth
Solid medium used to separate out microbes. ANSWER agar plate
Human flora bacteria used for investigations in osmotic pressuring, anti-bacterial
resistance, and biofilms. ANSWER S. epidermidis
Aggregation of genetically uniform microorganisms. ANSWER colony forming unit
Unicellular fungi used for studies related to fermentation, genetics, aging. ANSWER S.
cerevisiae
tool for streaking microorganisms onto a plate. - ANSWER Inoculation loop
1. Agar plates should be labeled on the _____. - ANSWER bottom
2. Aseptic techniques are done to ______________.
a. keep from harm
b. provide pure cultures
c. prevent contamination from unwanted microbes
d. All of the above - ANSWER d. all of the above
Agar is a solid media used in slants and plates.
,True
False - ANSWER true
4. The inoculation loop should be sterilized _____ when making a streak plate.
-before use
-between use in each quadrant
-after completing all quadrants
-All of the above - ANSWER all of the above
Morphology is a technique used to identify microorganisms both with and without the
use of a microscope.
True
False - ANSWER true
6. _____ is a rod-shaped bacteria important to the study of genetics and evolution.
-Escherichia coli
-Saccharomyces cerevisiae
-Staphylococcus epidermidis
-all of the above - ANSWER E. coli
1. Aseptic technique involves _____ .
-working quickly and efficiently around open media containers
-not talking or breathing over open media containers
-washing hands before and after working with media
-All of the above - ANSWER all of the above
2. All equipment and work surfaces should be cleaned with a bleach solution after
contacting microbe cultures.
,True
False - ANSWER true
TSA stands for Tryptic Soy Agar and is a _____ media.
-general purpose liquid
-selective liquid
-general purpose solid
-None of the above - ANSWER general purpose solid
Streak plating is accomplished by pouring broth onto agar plates.
True
False - ANSWER false-Streak plating is accomplished by spreading a sample of bacteria
over the surface of an agar plate with an inoculation loop.
_____ is a common, harmless microbe used in laboratory studies.
-Escherichia coli
-Saccharomyces cerevisiae
-Staphylococcus epidermidis
-All of the above - ANSWER all of the above
When the inoculation loop is transferring microbes from a broth into a plate, the
inoculation loop should be sterilized ____.
-before placing in broth
-after spreading microbes on the agar surface
-before disposal
-All of the above - ANSWER all of the above
Turbidity and flocculence are indicative of microbial growth in liquid media.
, True
False - ANSWER true
A pure plated culture should display colony forming units with two or more
morphologies.
True
False - ANSWER false-should be identical morphologies
Colony forming unit ANSWER describes individual colonies on a plate's surface The
number of individuals doubles each generation ANSWER exponential growth Cloudiness
of a fluid caused by large numbers of particles/organisms ANSWER turbidity The
measure of the light intensity absorbed by a sample ANSWER absorbance Asexual
reproduction resulting in two daughter cells ANSWER binary fission
Binary fission is a method of sexual reproduction where one parent cell will split into
four cells.
True
False - ANSWER false-Binary fission is the process of asexual reproduction in which a
single cell will divide to produce two daughter cells.
Plates with _____ colonies per plate are used to calcuate CFUs.
-10-30
-30-300
-20-100
-None of the above - ANSWER -30-300
To determine the number of CFUs present in the original culture, the number of colonies
on the viable plate is _____ the total dilution factor of that plate.
-added to
-multiplied by
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