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CSMLS Exam Questions And Answers 100% Pass

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CSMLS Exam Questions And Answers 100% Pass What molecule is responsible for HOW living organsims synthesize energy?` - answerDNA What are the 2 divisions of metabolism? - answerCatabolism and Anabolism What is the function of ATP? - answerStores energy for metabolism What are the four phases o...

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  • November 11, 2024
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  • CSMLS
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©THEBRIGHT EXAM SOLUTIONS

11/8/2024 12:08 PM


CSMLS Exam Questions And Answers 100%
Pass


What molecule is responsible for HOW living organsims synthesize energy?` - answer✔DNA

What are the 2 divisions of metabolism? - answer✔Catabolism and Anabolism

What is the function of ATP? - answer✔Stores energy for metabolism

What are the four phases of a bacterial growth curve? - answer✔Lag, Exponential, Stationary and Death

Define Sterilization - answer✔The destruction of all microbial life

Define Disinfection - answer✔The destruction of microbial life on an inanimate object

Define Antisepsis - answer✔The destruction of microbial life on living tissue (endospores may survive)

What is level 1 control of precautions? - answer✔Standard precautions - everyone should follow.

What are level 2 control of precautions? - answer✔Contact Precautions - when a patient is infected and
you can garb up to protect yourself because the infection can be spread with direct contact

What are level 3 control of precautions? - answer✔Droplet Precautions - patient can spread the
infection through droplets

What are level 4 control of precautions? - answer✔Airborne/ Isolation precautions. Can be spread
because microorganisms can survive and "fly" in air.

Vertical Gene Transfer - answer✔Genetics passed on through reproduction

Horizontal Gene Transfer - answer✔Transferring genes to another microbe of the same generation (sets
microbes away from multi-celled life)

What are the three mechanisms of horizontal gene transfer? - answer✔Transformation

Conjugation

Transduction

How can horizontal gene transfer change microorganisms? - answer✔Microbes can become more
virulent and drug resistant which would make the disease harder to treat

, ©THEBRIGHT EXAM SOLUTIONS

11/8/2024 12:08 PM

Transduction - answer✔Genetic material passes via a bacterial virus known as a bacteriophage

Transformation - answer✔Bacteria bind DNA from there surroundings (DNA could be floating due to
lysis)

Conjugation - answer✔Donor contracts recipient through a sex pilis. This results in both cells having
identical copies of the genetic material

Transposition - answer✔DNA pieces can hop to new locations on a genome

How can genetic diversity be studied? - answer✔By analyzing the sequence of nucleotides in rRNA

What type of ribosomes are in eukaryotic cells? - answer✔80S

What kind of ribosomes are within prokaryotic cells - answer✔70S

Clone vs. Strain - answer✔Clone is a population of cells derived from a single cell



Strain is genetically different cells within a clone

How are wavelength and resolution related in microscopy? - answer✔As the wavelength decreases,
resolution increases.

Resolution - answer✔To see 2 points as separate and distinct

What does it mean if a microscope has a resolving power of 0.4 nm? - answer✔That the microscope can
distinguish 2 points 0.5 nm apart

Refractive Index - answer✔The light bending ability of a medium (light may bend too much and miss the
high magnification lens)



Low refractive index = high resolution

What is the purpose of Immersion Oil - answer✔Is used to keep light from bending

What light is collected in Bright field Microscopes? - answer✔Both light reflected from the object and
unreflected lightq

What light is collected in Darkfeild Microscopes? - answer✔Only the light reflected from the object.



An opaque disc UNDER the condenser blocks out light that would otherwise enter the objective directly.

What is the purpose of Phase-Contrast Microscopy - answer✔Allows for detailed examination of internal
structures of non-fixed and unstained specimens

, ©THEBRIGHT EXAM SOLUTIONS

11/8/2024 12:08 PM

In-Phase vs. Out of Phase - answer✔In phase = peaks match and troughs match = adds light waves
together



Out of phase = peaks and troughs do not match up = cancels light waves out showing black in the
specimen since there is no light.

How does Florescent Microscopy work? - answer✔Florescent substances adsorb UV light and emit
visible light. They luminesce.

What is immunofluorescence? - answer✔With the use of a fluorochrome labelled AB in the diagnosis of
a specific infectious disease

How can specimens be fixed to a slide before staining? - answer✔With heat or methyl alcohol

Why do Acid- Fast bacteria retain the red carbolfuscin dye? - answer✔Because they have a waxy layer of
mycolic acid. The dye is more soluble in the lipid layer of the bacteria instead of the acid alcohol reagent

Psychrophiles - answer✔Prefer 0-20oC. They live in cold regions of the world and are not associated
with human disease

Thermophiles - answer✔Cannot grow at body temp (body temp is too low)

Mesophiles - answer✔Human pathogens. They grow at 20-40oC

What makes up ambient air - answer✔Naturally occurring gases in the air we breathe.



21% O2

0.03% CO2

78% N2

And a mix of other gases

What makes up a microaerophilic environment? - answer✔5% O2

10%CO2

85% N2



*needed for Campy organisms.... O2 levels higher than 6% will harm these organisms

What is Diamond Blackfan Anemia? - answer✔The congenital form of red cell aplasia that occurs due to
a mutation of ribosomes.

, ©THEBRIGHT EXAM SOLUTIONS

11/8/2024 12:08 PM


Short stature, neck and thumb abnormalities

Macrocytic anemia

Reticulocytopenia

NL - Decreased WBC

NL PLTS

What is the cause of anemia with Congenital Dyserythropoietic Anemia? - answer✔Hypercellulary BM
with ineffective erythropoiesis - days plastic changes

What is noted from CDA I? - answer✔Spongy hemochromatin (Swiss cheese) in erythroblasts



May show nuclear bridging from chromatin bridges

-basophillic stippling and Cabot rings

What is noted in CDA II? - answer✔Binucleated and multinucleated forms (pseudo gaucher cells)



Can be treated with a splenectomy and iron depletion

What is noted in CDA III? - answer✔BM will show giant erythroblasts with up to 12 nuclei

What causes myelophthisic anemia - answer✔Infiltration of abnormal cells (cancer cells, tumours,
fibroblasts) into the BM, releasing immature cells into circulation.



Stem cells and progenitors migrate to the spleen and liver for extra medullary hematopoiesis

What does the PB of Myelophthisic Anemia appear as? - answer✔Normocytic erythrocytes

Reticulocytopenia

Tear drop cells

NRBC's

Immature myeloid and megakaryocytes

Giant PLTS

What causes Anemia of Chronic Kidney Disease? - answer✔Inadequate production of EPO

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