ASCP Hematology Review
Name the locations of active marrow in adults: - answersternum, skull, ribs, pelvis, vertebrae
Where does hematopoiesis take place during the first 2 months of gestation? - answeryolk
sac
Where does the majority of hematopoiesis take place during the 2nd through 7th month of
gestation? - answerspleen
When does the bone marrow take over hematopoiesis? - answerafter birth
What are the stages in order of the erythrocyte lineage? - answer(1)rubriblast,
(2)prorubricyte, (3)rubricyte, (4)metarubricyte, (5)reticulocyte, (6)erytrocyte
What are the stages in order of the granulocyte (neutrophil, eosinophil, basophil) lineage? -
answer(1)myeloblast, (2)promyelocyte, (3)myelocyte, (4)metamyelocyte, (5)band,
(6)segmented (7)eos/baso
What are the stages in order of the macrophage lineage? - answer(1)monoblast,
(2)promonocyte, (3)monocyte, (4)macrophage
What are the stages in order of the platelet lineage? - answer(1)megakaryoblast,
(2)promegakaryocyte, (3)megakaryocyte, (4)platelet
What are the stages in order of the lymphocyte lineage? - answer(1)lymphoblast,
(2)prolymphocyte, (3)lymphocyte
Which growth factors stimulate the erythrocyte lineage? - answergm-CSF, EPO, IL-3
Which growth factors stimulate the granulocyte lineages? - answergm-CSF, IL-3
Which growth factors stimulate the platelet lineage? - answergm-CSF, IL-3, TPO
Which growth factors stimulate the lymphocyte lineage? - answeril-2, IL-4, IL-6, IL-7
Which protein functions in the transport of Fe? - answertransferrin
What is the major storage form of Fe? - answerferritin
What is the long-term (H20) insoluble Fe storage form called? - answerhemosiderin
Excess Iron is stored where? What does this cause? - answertissues and body organs,
hemosiderosis, hemochromatosis
Porphyrias are attributed to? - answerexcessive formation of porphrins due to blockages in
any enzymatic step in the heme synthesis pathway.
Red or port wine colored urine can be seen in which condition related to the build up of
heme precursors? - answerporphyria
Neuropsychiatirc symptoms are seen when a build up of which precursors occurs? -
answerearly precursors (Delta-aminolevulinic acid/Porphobilinogen)
, Cutaneous symptoms such as photo-sensitivity and facial hair are seen when a build up of
which precursors occurs? - answerlater precursors (uroporphyrinogen/coproporphyrinogen,
protoporphyrin/heme)
Synthesis of heme requires what two components? - answeriron, protoporphyrin
What are the heme precursors in order of synthesis? - answer(1)delta-aminolevulinic acid,
(2)porphobilinogen, (3)uroporphyrinogen, (4)coproporphyrinogen, (5)protoporphyrin,
(6)heme
What type of hemoglobin is seen primarily in newborns? - answerhgb F
What type of hemoglobin is seen primarily in adults? - answerhgb A2
What type of hemoglobin is rarely seen in adults as well as newborns? - answerhgb A
What hemoglobin types are seen in embryos but not in adults or newborns? - answergower I,
gower II, portland
All non-embryonic hemoglobin types are comprised of what type of globin chain? -
answeralpha
Hemoglobin A2 is composed of what types of globin chains? - answeralpha, beta
Hemoglobin A is composed of what types of globin chains? - answeralpha, delta
Hemoglobin F is composed of what types of globin chains? - answeralpha, gamma
Multiple transfusions can cause what kind of shift in the hemoglobin-oxygen dissociation
curve? - answerleft shift
An increase in hemoglobin F can cause what kind of shift in the hemoglobin-oxygen
dissociation curve? - answerleft shift
A left shift in the hemoglobin-oxygen dissociation curve causes what changes in the
following substances: CO2, ph, 2,3-DPG? - answerincreased ph, decreased CO2, decreased
2,3-DPG
A right shift in the hemoglobin-oxygen dissociation curve causes what changes in the
following substances: CO2, ph, 2,3-DPG? - answerdecreased ph, increased CO2, increased
2,3-DPG
How does EDTA work as an anticoagulant? - answerit chelates Ca++
How does heparin work as an anticoagulant? - answerit is an anti-thrombin agent
Hemoglobin is measured by its conversion to what? By? - answercyanomethemoglobin,
potassium cyanide, potassium ferricyanide
What are potential sources of error in the measurement of hemoglobin? - answerlipemia,
icterus, high white count, resistant hemoglobins (SS, CC)
What is the normal reference range of hemoglobin for males? - answer14-18 g/dl
What is the normal reference range of hemoglobin for females? - answer12-16 g/dl
