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BACE 4.0 - Laboratory Skills-Applications Questions and Answers 2024
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BACE 4.0 - Laboratory Skills-Applications
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BACE 4.0 - Laboratory Skills-Applications
why must technicians practice aseptic technique when culturing cells? - answer to
ensure they are growing pure cultures of their desired cells.
which of the following techniques destroys all microorganisms? - answer sterilization
when using a microscope on the highest power objective, which focus knob should you
use? - answer Fine
a solution with a pH of 3.0 is how many times more acidic than a solution with a pH of
6.0? - answer1000
salt (such as sodium chloride or sodium acetate in) is commonly used in procedures to
extract plasmid DNA. which of the following best describes the role of the salt? - answer
the positively charged sodium ions neutralize the negative charge on the DNA.
why is plasmid DNA is purified using an ion exchange column chromatography? -
answerDNA is negatively charged, binds to the positively charged resin, and is eluded
with increasing salt.
a technician is testing the purity of a DNA sample using a spectrophotometer. he gets a
260 nm reading of 0.51 au, and a 280 nm reading of .65 au. recalling that the preferred
range of the value is 1.8 to 2.0, is the purity of the sample acceptable? - answerno
when using an acrylamide gel for protein electrophoresis, it is best to use a higher
percentage of acrylamide to resolve smaller proteins, while larger proteins are best
results on low percent gels. - answerTrue
staining with Coomassie blue - answerto visualize proteins after PAGE
adding loading dye to protein samples - answerto facilitate loading samples into Wells,
and to monitor sample migration during page
adding a molecular weight standard to an empty well - answerto determine the relative
sizes of the samples run on the gel
adding SDS to the sample buffer and the page running buffer - answerto coat the
sample proteins with a negative charge, and denature them
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