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BACE Exam practice. Multiple Choice 2024 Questions and Answers Rated A+
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BACE Exam practice. Multiple Choice 2024
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BACE Exam practice. Multiple Choice2024
how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - answer(50 mL)
(5 mg,mL) = 250 mg
a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately
be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the
correct order the technician must perform them to accomplish her task. - answer1)
design primers to amplify the lanA sequence using polymerase chain reaction (PCR)
and perform the PCR reaction
2) digest and provide lanA PCR product and the plasmid with appropriate restriction
enzymes
3) use ligase to "paste" LanA into a plasmid
4) transform E. coli with plasmid containing LanA
how much tetracycline is needed to prepare a 100 mL of 100 mg /ml tetracycline (w/v)?
- answer(100mL)(100mg/ML) = 10000mg (10g)
after performing the PCR reaction with the primers she designed to amplify lanA, the
same technician found her PCR reaction contained two distinct bands, one at the
expected size of 1200 kilobase pairs, and another unexpected band at 2000 Kilobase
pairs. what is the most likely reason there's an unexpected bands and her PCR
reaction? - answershe used an annealing temperature that was too low
in a biotechnology lab, tap water is best to use for: - answerfirst rinse when washing
glassware
the order of amino acids on a polypeptide chain is encoded by: - answermRNA
how do DNA molecules vary from one species to another? - answerthe number of
genes and non-coding regions
what is agar? - answersolid media on which bacteria grows
what part of an amino acid differs from one to the other? - answerR group
, how are the proteins in fractions collected during column chromatography visualized? -
answerusing polyacrylamide gel electrophoresis (SDS PAGE)
which of the following is an mRNA codon? - answerUAU
what is the purpose of SDS when used in SDS page electrophoresis? - answerdenature
the proteins and give them a negative charge
which department is in charge of testing biopharmaceutical products to ensure its safety
and potency? - answerquality control (QC)
lipids provide which of the following functions for cells? - answerstored energy
which of the following is a monosaccharide commonly used as an energy source for
cells in cell culture media? - answerglucose
where does translation occur in prokaryotes? - answerat the ribosomes
in aerobic respiration carbohydrates are ultimately broken down into: - answerCO2,
H2O, ATP
most ATP in eukaryotic cells is produced in the: - answermitochondria
an aqueous solution and yields a hydrogen ion when ionized - answeracid
defined as "the moles of solute per liter of solution" - answermolarity
a solution that resists changes in PH - answerbuffer
the measurement of the concentration of hydrogen ion in a sample - answerpH
type of test to measure the amount of total protein present - answerconcentration
assays
type of test used to measure the amount of active ingredient in a drug - answerpotency
assays
type of test that demonstrates whether or not a specific enzyme or drug is having the
desired effect for which it was designed - answeractivity assays
a highly specific test to determine protein concentration based on a particular antigen
antibody interaction - answerELISA
a document listing this detailed steps of key processes to help ensure consistent and
high-quality output - answerSOP
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