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BACE test Questions and Answers Graded A+
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Exam of 6 pages for the course BACE at BACE (BACE test)
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BACE testA technician is assigned the task of inserting a gene, lanA, into a plasmid to ultimately
be expressed
as a therapeutic protein in E. coli. Label the following steps (1-4) in the correct order the
technician
must perform them to accomplish her task.
Transform E. coli with the plasmid containing lanA.
Digest the amplified lanA PCR product and plasmid with appropriate restriction
enzymes.
Design primers to amplify the lanA sequence using polymerase chain reaction (PCR),
and perform the PCR reaction.
Use ligase to "paste" lanA into a plasmid. - answer4 2 1 3
After performing the PCR reaction with the primers she designed to amplify lanA, the
same
technician found that her PCR reaction contained two distinct bands, one at the
expected size of
1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the
most likely
reason there is an unexpected band in her PCR reaction?
a. She added too many nucleotides
b. She added too much polymerase
c. She used an annealing temperature that was too low
d. She used an annealing temperature that was too high - answerc She used an
annealing temperature that was too low
In a biotechnology lab, tap water is best used for:
a) Making buffers
b) The first rinse when washing glassware
c) The final rinse when washing glassware
d) All of the above - answerb The first rinse when washing glassware
The order of amino acids on a polypeptide chain is encoded by:
a) DNA
b) mRNA
c) rRNA
d) Regulatory proteins - answerb) mRNA
, How do DNA molecules vary from one species to another?
a) The type of nitrogenous bases they contain
b) The number of genes and non-coding regions
c) The way nitrogenous bases pair
d) The directionality of the strand - answerb) The number of genes and non-coding
regions
What is agar?
a) Solid media on which bacteria grow
b) Liquid media in which bacterial grow
c) Solid matrix used for DNA gel electrophoresis
d) Solid matrix used for vertical gel electrophoresis - answera) Solid media on which
bacteria grow
What part of an amino acid differs from one to the other?
a) Amino group
b) Carboxyl group
c) R group
d) All of the above - answerc) R group
How are the proteins in fractions collected during column chromatography visualized?
a) Using a pH meter
b) Using agarose gel electrophoresis
c) Using polyacrylamide gel electrophoresis (SDS PAGE)
d) Using microscopy - answerc) Using polyacrylamide gel electrophoresis (SDS PAGE)
Which of the following is an mRNA codon?
a) ATA
b) UAU
c) Tyrosine
d) All of the above - answerb) UAU
What is the purpose of SDS when used in SDS PAGE electrophoresis?
a) Stain the proteins for visualization
b) Use as a loading dye
c) Denature the proteins and give them a negative charge
d) Conduct electricity - answerc) Denature the proteins and give them a negative charge
Which department is in charge of testing a biopharmaceutical product to ensure its
safety and
potency?
a) Research and Development (R&D)
b) Quality Assurance (QA)
c) Manufacturing
d) Quality Control (QC) - answerd) Quality Control (QC)
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