Bio 219- Quizzes Questions With Complete Solutions
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Course
BIO 219
Institution
BIO 219
Bio 219- Quizzes
Questions With Complete Solutions
Which gene in the lux operon encodes for the transcriptional activator that binds the auto
inducer?
a. Lux E
b. Lux A
c. Lux D
d. Lux R
e. Lux I D. Lux R
The Aliivibrio fischeri strain we are using the term (MJ 1741) comes from the pineap...
Bio 219- Quizzes
Questions With Complete Solutions
Which gene in the lux operon encodes for the transcriptional activator that binds the auto
inducer?
a. Lux E
b. Lux A
c. Lux D
d. Lux R
e. Lux I D. Lux R
The Aliivibrio fischeri strain we are using the term (MJ 1741) comes from the pineapple fish
(Monocentris japonica). Also known as the pineconefish, it is yellow with distinct large scales
outlined in black. The fish is nocturnal and feeds at night. During the day, it shelters in caves and
under ledges. It has light-producing organs filled with the bioluminescent bacteria on each side
of its lower jaw. Though the purpose of these photo-organs is not specifically known, what do
you think may be the benefit to the host organism?
a. Attracting prey
b. Attracting a mate
c.Hiding from predators
d.Communication A. attracting prey
, Bio 219- Quizzes
Questions With Complete Solutions
High level of [x] activates the operon systems of luminous bacteria like A. fischeri in its
environment. The regulatory response to this molecule leads to the induction of expression of the
lux genes which is termed [y]. Therefore, where is the bacteria is most likely to glow? Inside its
host or in the open ocean? [z] x: autoinducer (AHL)
y: quorum sensing
z: inside its host
What is used to elute the DNA from the silica membrane in the spin column?
a. SDS (sodium dodecyl sulfate)
b.Water
c.Ethanol
d.A low pH buffer
e.Chaotropic salts B. water
What is the range for the 260:280 ratio that indicates a DNA sample of "good purity"?
a.0.5 - 1.5
b.2.0 - 2.2
, Bio 219- Quizzes
Questions With Complete Solutions
c.2.2 - 3.5
d.1.8 - 2.0
e.1.5 - 1.8 D. 1.8-2.0
A 260:280 ratio = 1.66 would indicate protein contamination. (True/False) True
If you wanted to get a brighter bioluminescence from your culture of Aliivibrio which of the
following "treatments" would increase the amount of blue-green light emitted?
a.Treatment with acid
b.Treatment with O2
c.Treatment with CO2
d.Treatment with chaotropic salts
e.Treatment with a alkaline buffer B. Treatment with 02
Wash Buffer 1 (AW1) contains low amounts of ________________ that binds to and removes
the proteins and colored contaminants.
a.Ethanol
b.SDS
, Bio 219- Quizzes
Questions With Complete Solutions
c.Guanidinium chloride
d.Sodium benzoate
e.Hydrochloric acid C. Guanidinum Chloride
The Resuspension Buffer contains ____________ that can solubilize the membrane proteins and
disrupt the lipid bilayer of gram-negative cells to help get the proteins into solution. The
phospholipids in the membrane are solubilized by this reagent. It also helps release the DNA by
binding proteins (both membrane and non-membrane proteins) and denaturing them..
A. SBS (sodium benzoate salt)
b.Ethanol
c.Silica
d.SDS (sodium dodecyl sulfate)
e.Chaotropic salts D. SDS (sodium dodecyl sulfate
On the spin column, the formation of an anionic bridge allows DNA to bind to the silica
membrane in the column. (True/False) False
The maximum wavelength of DNA absorbance is 260nm, within the UV range. This is due to the
nitrogenous bases of DNA (adenine, cytosine, guanine, and thymine). At this wavelength, double
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